Berndsen, Z., Bowman, C., Jang, H., & Ward, A. B. (2017). EMHP: an accurate automated hole masking algorithm for single-particle cryo-EM image processing. Bioinformatics, 33(23), 3824-3826.
The Electron Microscopy Hole Punch (EMHP) is a streamlined suite of tools for quick assessment, sorting and hole masking of electron micrographs. With recent advances in single-particle electron cryo-microscopy (cryo-EM) data processing allowing for the rapid determination of protein structures using a smaller computational footprint, we saw the need for a fast and simple tool for data pre-processing that could run independent of existing high-performance computing (HPC) infrastructures. EMHP provides a data preprocessing platform in a small package that requires minimal python dependencies to function.