Cassidy, C. K.; Himes, B. A.; Luthey-Schulten, Z. & Zhang, P. CryoEM-based hybrid modeling approaches for structure determination. Current opinion in microbiology, 2018, 43, 14-23
ecent advances in cryo-electron microscopy (cryoEM) have dramatically improved the resolutions at which vitrified biological specimens can be studied, revealing new structural and mechanistic insights over a broad range of spatial scales. Bolstered by these advances, much effort has been directed toward the development of hybrid modeling methodologies for the construction and refinement of high-fidelity atomistic models from cryoEM data. In this brief review, we will survey the key elements of cryoEM-based hybrid modeling, providing an overview of available computational tools and strategies as well as several recent applications.