2021Terwilliger CryoID

From 3DEM-Methods
Jump to: navigation, search

Citation

Terwilliger, T. C.; Sobolev, O. V.; Afonine, P. V.; Adams, P. D.; Ho, C. M.; Li, X. & Zhou, Z. H. Protein identification from electron cryomicroscopy maps by automated model building and side-chain matching. Acta crystallographica. Section D, Structural biology, 2021, 77, 457-462

Abstract

Using single-particle electron cryo-microscopy (cryo-EM), it is possible to obtain multiple reconstructions showing the 3D structures of proteins imaged as a mixture. Here, it is shown that automatic map interpretation based on such reconstructions can be used to create atomic models of proteins as well as to match the proteins to the correct sequences and thereby to identify them. This procedure was tested using two proteins previously identified from a mixture at resolutions of 3.2 Å, as well as using 91 deposited maps with resolutions between 2 and 4.5 Å. The approach is found to be highly effective for maps obtained at resolutions of 3.5 Å and better, and to have some utility at resolutions as low as 4 Å.

Keywords

Links

https://scripts.iucr.org/cgi-bin/paper?ir5016

Related software

Related methods

Comments