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2010Zhang OpNS - Revision history
2024-03-28T17:46:18Z
Revision history for this page on the wiki
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http://3demmethods.i2pc.es/index.php?title=2010Zhang_OpNS&diff=3704&oldid=prev
Garygangren: /* Related methods */
2020-06-30T14:28:48Z
<p><span dir="auto"><span class="autocomment">Related methods</span></span></p>
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<td colspan='2' style="background-color: white; color:black; text-align: center;">Revision as of 14:28, 30 June 2020</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://pubmed.ncbi.nlm.nih.gov/20978167/</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://pubmed.ncbi.nlm.nih.gov/20978167/</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://pubmed.ncbi.nlm.nih.gov/25145703/</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://pubmed.ncbi.nlm.nih.gov/25145703/</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://pubmed.ncbi.nlm.nih.gov/20978167/</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://pubmed.ncbi.nlm.nih.gov/20978167/</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://www.springer.com/gp/book/9781627032742</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://www.springer.com/gp/book/9781627032742</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://pubmed.ncbi.nlm.nih.gov/23032862/</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://pubmed.ncbi.nlm.nih.gov/23032862/</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Comments ==</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Comments ==</div></td></tr>
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Garygangren
http://3demmethods.i2pc.es/index.php?title=2010Zhang_OpNS&diff=3703&oldid=prev
Garygangren: /* Links */
2020-06-30T14:28:31Z
<p><span dir="auto"><span class="autocomment">Links</span></span></p>
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<td colspan='2' style="background-color: white; color:black; text-align: center;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black; text-align: center;">Revision as of 14:28, 30 June 2020</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://pubmed.ncbi.nlm.nih.gov/19965615/</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://pubmed.ncbi.nlm.nih.gov/19965615/</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://www.ncbi.nlm.nih.gov/pubmed/29129068</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://www.ncbi.nlm.nih.gov/pubmed/29129068</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>http://www.ncbi.nlm.nih.gov/pubmed/20978167</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>http://www.ncbi.nlm.nih.gov/pubmed/20978167</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>http://www.nature.com/articles/ncomms11083</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>http://www.nature.com/articles/ncomms11083</div></td></tr>
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Garygangren
http://3demmethods.i2pc.es/index.php?title=2010Zhang_OpNS&diff=3702&oldid=prev
Garygangren: /* Links */
2020-06-30T14:28:08Z
<p><span dir="auto"><span class="autocomment">Links</span></span></p>
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<td colspan='2' style="background-color: white; color:black; text-align: center;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black; text-align: center;">Revision as of 14:28, 30 June 2020</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Links ==</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Links ==</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">https://www.jove.com/video/51087/optimized-negative-staining-high-throughput-protocol-for-examining</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://pubmed.ncbi.nlm.nih.gov/19965615/</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://pubmed.ncbi.nlm.nih.gov/19965615/</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://www.ncbi.nlm.nih.gov/pubmed/29129068</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://www.ncbi.nlm.nih.gov/pubmed/29129068</div></td></tr>
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Garygangren
http://3demmethods.i2pc.es/index.php?title=2010Zhang_OpNS&diff=3701&oldid=prev
Garygangren: /* Abstract */
2020-06-30T14:27:20Z
<p><span dir="auto"><span class="autocomment">Abstract</span></span></p>
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<td colspan='2' style="background-color: white; color:black; text-align: center;">Revision as of 14:27, 30 June 2020</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Abstract ==</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Abstract ==</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del class="diffchange diffchange-inline">Apolipoprotein E (apoE), one </del>of <del class="diffchange diffchange-inline">the major protein components </del>of <del class="diffchange diffchange-inline">lipoproteins in the peripheral </del>and <del class="diffchange diffchange-inline">central nervous systems, regulates cholesterol metabolism through its interaction </del>with <del class="diffchange diffchange-inline">members of the low density lipoprotein receptor family. One key to understanding apoE function </del>is <del class="diffchange diffchange-inline">determining the structure of lipid-bound forms of apoE</del>. Negative<del class="diffchange diffchange-inline">-</del>staining (NS) electron microscopy (EM) is an easy and <del class="diffchange diffchange-inline">rapid </del>approach <del class="diffchange diffchange-inline">for studying the </del>structure and <del class="diffchange diffchange-inline">morphology of lipid</del>-<del class="diffchange diffchange-inline">bound forms of apoE</del>. <del class="diffchange diffchange-inline">However</del>, <del class="diffchange diffchange-inline">an artifact of using the </del>conventional NS <del class="diffchange diffchange-inline">protocol is </del>that <del class="diffchange diffchange-inline">the apoE phospholipid particles </del>form rouleaux. <del class="diffchange diffchange-inline">In this study, we used </del>cryo-electron microscopy (cryo-EM) <del class="diffchange diffchange-inline">to examine apoE4 palmitoyl-oleoylphosphatidylcholine </del>(<del class="diffchange diffchange-inline">POPC</del>) <del class="diffchange diffchange-inline">particles in </del>a <del class="diffchange diffchange-inline">frozen</del>-<del class="diffchange diffchange-inline">hydrated native state</del>. <del class="diffchange diffchange-inline">By comparing </del>the <del class="diffchange diffchange-inline">particle sizes and shapes produced by different NS protocols to those produced by cryo</del>-<del class="diffchange diffchange-inline">EM, we propose an optimized protocol </del>to examine <del class="diffchange diffchange-inline">apoE4 POPC particles</del>. <del class="diffchange diffchange-inline">Statistical analysis demonstrated that </del>the <del class="diffchange diffchange-inline">particle sizes differ by less than 5% between </del>the <del class="diffchange diffchange-inline">optimized protocol </del>and <del class="diffchange diffchange-inline">the </del>cryo-EM method<del class="diffchange diffchange-inline">, with similar shapes</del>. <del class="diffchange diffchange-inline">The high contrast and fine detail of particle images produced using this optimized </del>protocol <del class="diffchange diffchange-inline">lend themselves </del>to the <del class="diffchange diffchange-inline">structural study </del>of <del class="diffchange diffchange-inline">lipid-bound forms of apoE</del>.</div></td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins class="diffchange diffchange-inline">Structural determination </ins>of <ins class="diffchange diffchange-inline">proteins is rather challenging for proteins with molecular masses between 40 - 200 kDa. Considering that more than half </ins>of <ins class="diffchange diffchange-inline">natural proteins have a molecular mass between 40 - 200 kDa, a robust </ins>and <ins class="diffchange diffchange-inline">high-throughput method </ins>with <ins class="diffchange diffchange-inline">a nanometer resolution capability </ins>is <ins class="diffchange diffchange-inline">needed</ins>. Negative staining (NS) electron microscopy (EM) is an easy<ins class="diffchange diffchange-inline">, rapid, </ins>and <ins class="diffchange diffchange-inline">qualitative </ins>approach <ins class="diffchange diffchange-inline">which has frequently been used in research laboratories to examine protein </ins>structure and <ins class="diffchange diffchange-inline">protein</ins>-<ins class="diffchange diffchange-inline">protein interactions</ins>. <ins class="diffchange diffchange-inline">Unfortunately</ins>, conventional NS <ins class="diffchange diffchange-inline">protocols often generate structural artifacts on proteins, especially with lipoproteins </ins>that <ins class="diffchange diffchange-inline">usually </ins>form <ins class="diffchange diffchange-inline">presenting </ins>rouleaux <ins class="diffchange diffchange-inline">artifacts</ins>. <ins class="diffchange diffchange-inline">By using images of lipoproteins from </ins>cryo-electron microscopy (cryo-EM) <ins class="diffchange diffchange-inline">as a standard, the key parameters in NS specimen preparation conditions were recently screened and reported as the optimized NS protocol </ins>(<ins class="diffchange diffchange-inline">OpNS</ins>)<ins class="diffchange diffchange-inline">, </ins>a <ins class="diffchange diffchange-inline">modified conventional NS protocol. Artifacts like rouleaux can be greatly limited by OpNS, additionally providing high contrast along with reasonably high</ins>-<ins class="diffchange diffchange-inline">resolution (near 1 nm) images of small and asymmetric proteins</ins>. <ins class="diffchange diffchange-inline">These high-resolution and high contrast images are even favorable for an individual protein (a single object, no average) 3D reconstruction, such as a 160 kDa antibody, through </ins>the <ins class="diffchange diffchange-inline">method of electron tomography. Moreover, OpNS can be a high</ins>-<ins class="diffchange diffchange-inline">throughput tool </ins>to examine <ins class="diffchange diffchange-inline">hundreds of samples of small proteins</ins>. <ins class="diffchange diffchange-inline">For example, </ins>the <ins class="diffchange diffchange-inline">previously published mechanism of 53 kDa cholesteryl ester transfer protein (CETP) involved </ins>the <ins class="diffchange diffchange-inline">screening </ins>and <ins class="diffchange diffchange-inline">imaging of hundreds of samples. Considering </ins>cryo-EM <ins class="diffchange diffchange-inline">rarely successfully images proteins less than 200 kDa has yet to publish any study involving screening over one hundred sample conditions, it is fair to call OpNS a high-throughput </ins>method <ins class="diffchange diffchange-inline">for studying small proteins</ins>. <ins class="diffchange diffchange-inline">Hopefully the OpNS </ins>protocol <ins class="diffchange diffchange-inline">presented here can be a useful tool </ins>to <ins class="diffchange diffchange-inline">push </ins>the <ins class="diffchange diffchange-inline">boundaries </ins>of <ins class="diffchange diffchange-inline">EM and accelerate EM studies into small protein structure, dynamics and mechanisms</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Keywords ==</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Keywords ==</div></td></tr>
</table>
Garygangren
http://3demmethods.i2pc.es/index.php?title=2010Zhang_OpNS&diff=3700&oldid=prev
Garygangren: /* Citation */
2020-06-30T14:26:48Z
<p><span dir="auto"><span class="autocomment">Citation</span></span></p>
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<td colspan='2' style="background-color: white; color:black; text-align: center;">Revision as of 14:26, 30 June 2020</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Citation ==</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Citation ==</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Rames, Matthew, Yu, Yadong, and Ren, Gang. Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy. United States: N. p., 2014. Web. doi:10.3791/51087</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>Zhang L, Song J, Newhouse Y, Zhang S, Weisgraber KH, Ren G. An optimized negative-staining protocol of electron microscopy for apoE4 POPC lipoprotein. J Lipid Res. 2010;51(5):1228-1236. doi:10.1194/jlr.D002493</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>Zhang L, Song J, Newhouse Y, Zhang S, Weisgraber KH, Ren G. An optimized negative-staining protocol of electron microscopy for apoE4 POPC lipoprotein. J Lipid Res. 2010;51(5):1228-1236. doi:10.1194/jlr.D002493</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
</table>
Garygangren
http://3demmethods.i2pc.es/index.php?title=2010Zhang_OpNS&diff=3674&oldid=prev
Garygangren: /* Links */
2020-06-29T20:59:47Z
<p><span dir="auto"><span class="autocomment">Links</span></span></p>
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<td colspan='2' style="background-color: white; color:black; text-align: center;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black; text-align: center;">Revision as of 20:59, 29 June 2020</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Links ==</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Links ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://pubmed.ncbi.nlm.nih.gov/19965615/</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>https://pubmed.ncbi.nlm.nih.gov/19965615/</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">https://www.ncbi.nlm.nih.gov/pubmed/29129068</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">http://www.ncbi.nlm.nih.gov/pubmed/20978167</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">http://www.nature.com/articles/ncomms11083</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Related software ==</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Related software ==</div></td></tr>
</table>
Garygangren
http://3demmethods.i2pc.es/index.php?title=2010Zhang_OpNS&diff=3668&oldid=prev
Garygangren: Created page with "== Citation == Zhang L, Song J, Newhouse Y, Zhang S, Weisgraber KH, Ren G. An optimized negative-staining protocol of electron microscopy for apoE4 POPC lipoprotein. J Lipid R..."
2020-06-29T20:19:26Z
<p>Created page with "== Citation == Zhang L, Song J, Newhouse Y, Zhang S, Weisgraber KH, Ren G. An optimized negative-staining protocol of electron microscopy for apoE4 POPC lipoprotein. J Lipid R..."</p>
<p><b>New page</b></p><div>== Citation ==<br />
Zhang L, Song J, Newhouse Y, Zhang S, Weisgraber KH, Ren G. An optimized negative-staining protocol of electron microscopy for apoE4 POPC lipoprotein. J Lipid Res. 2010;51(5):1228-1236. doi:10.1194/jlr.D002493<br />
<br />
== Abstract ==<br />
Apolipoprotein E (apoE), one of the major protein components of lipoproteins in the peripheral and central nervous systems, regulates cholesterol metabolism through its interaction with members of the low density lipoprotein receptor family. One key to understanding apoE function is determining the structure of lipid-bound forms of apoE. Negative-staining (NS) electron microscopy (EM) is an easy and rapid approach for studying the structure and morphology of lipid-bound forms of apoE. However, an artifact of using the conventional NS protocol is that the apoE phospholipid particles form rouleaux. In this study, we used cryo-electron microscopy (cryo-EM) to examine apoE4 palmitoyl-oleoylphosphatidylcholine (POPC) particles in a frozen-hydrated native state. By comparing the particle sizes and shapes produced by different NS protocols to those produced by cryo-EM, we propose an optimized protocol to examine apoE4 POPC particles. Statistical analysis demonstrated that the particle sizes differ by less than 5% between the optimized protocol and the cryo-EM method, with similar shapes. The high contrast and fine detail of particle images produced using this optimized protocol lend themselves to the structural study of lipid-bound forms of apoE.<br />
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== Keywords ==<br />
protein structure, lipoprotein structure, electron microscopy, negative-staining, optimized negative-staining protocol, individual-particle electron tomography<br />
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== Links ==<br />
https://pubmed.ncbi.nlm.nih.gov/19965615/<br />
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== Related software ==<br />
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== Related methods ==<br />
https://pubmed.ncbi.nlm.nih.gov/20978167/<br />
https://pubmed.ncbi.nlm.nih.gov/25145703/<br />
https://pubmed.ncbi.nlm.nih.gov/20978167/<br />
https://www.springer.com/gp/book/9781627032742<br />
https://pubmed.ncbi.nlm.nih.gov/23032862/<br />
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== Comments ==</div>
Garygangren