2014Scheres Movies

From 3DEM-Methods
Revision as of 17:21, 18 August 2014 by CoSS (talk | contribs) (Created page with "== Citation == Scheres, S. H. Beam-induced motion correction for sub-megadalton cryo-EM particles. Elife, 2014, 3, e03665 == Abstract == In electron cryo-microscopy (cryo-E...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

Citation

Scheres, S. H. Beam-induced motion correction for sub-megadalton cryo-EM particles. Elife, 2014, 3, e03665

Abstract

In electron cryo-microscopy (cryo-EM), the electron beam that is used for imaging also causes the sample to move. This motion blurs the images and limits the resolution attainable by single-particle analysis. In a previous Research article (Bai et al., 2013) we showed that correcting for this motion by processing movies from fast direct-electron detectors allowed structure determination to near-atomic resolution from 35,000 ribosome particles. In this Research advance article, we show that an improved movie processing algorithm is applicable to a much wider range of specimens. The new algorithm estimates straight movement tracks by considering multiple particles that are close to each other in the field of view, and models the fall-off of high-resolution information content by radiation damage in a dose-dependent manner. Application of the new algorithm to four data sets illustrates its potential for significantly improving cryo-EM structures, even for particles that are smaller than 200 kDa

Keywords

Links

http://www.ncbi.nlm.nih.gov/pubmed/25122622

Related software

Related methods

Comments