2016Wu Review
From 3DEM-Methods
Citation
Wu, S.; Armache, J.-P. & Cheng, Y. Single-particle cryo-EM data acquisition by using direct electron detection camera. Microscopy, 2015
Abstract
Recent advances in single-particle electron cryo-microscopy (cryo-EM) were largely facilitated by the application of direct electron detection cameras. These cameras feature not only a significant improvement in detective quantum efficiency but also a high frame rate that enables images to be acquired as ‘movies’ made of stacks of many frames. In this review, we discuss how the applications of direct electron detection cameras in cryo-EM have changed the way the data are acquired.
Keywords
Links
http://www.ncbi.nlm.nih.gov/pubmed/26546989
