2018Afonine Phenix

From 3DEM-Methods
Revision as of 07:08, 27 June 2019 by WikiSysop (talk | contribs) (Created page with "== Citation == Afonine, P. V.; Klaholz, B. P.; Moriarty, N. W.; Poon, B. K.; Sobolev, O. V.; Terwilliger, T. C.; Adams, P. D. & Urzhumtsev, A. New tools for the analysis and...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

Citation

Afonine, P. V.; Klaholz, B. P.; Moriarty, N. W.; Poon, B. K.; Sobolev, O. V.; Terwilliger, T. C.; Adams, P. D. & Urzhumtsev, A. New tools for the analysis and validation of cryo-EM maps and atomic models. Acta crystallographica. Section D, Structural biology, 2018 , 74 , 814-840

Abstract

Recent advances in the field of electron cryomicroscopy (cryo-EM) have resulted in a rapidly increasing number of atomic models of biomacromolecules that have been solved using this technique and deposited in the Protein Data Bank and the Electron Microscopy Data Bank. Similar to macromolecular crystallography, validation tools for these models and maps are required. While some of these validation tools may be borrowed from crystallography, new methods specifically designed for cryo-EM validation are required. Here, new computational methods and tools implemented in PHENIX are discussed, including d to estimate resolution, phenix.auto_sharpen to improve maps and phenix.mtriage to analyze cryo-EM maps. It is suggested that cryo-EM half-maps and masks should be deposited to facilitate the evaluation and validation of cryo-EM-derived atomic models and maps. The application of these tools to deposited cryo-EM atomic models and maps is also presented.

Keywords

Links

https://www.ncbi.nlm.nih.gov/pubmed/30198894

Related software

Related methods

Comments