http://3demmethods.i2pc.es/index.php?title=2020Yoder_TimeResolved&feed=atom&action=history2020Yoder TimeResolved - Revision history2024-03-28T10:07:27ZRevision history for this page on the wikiMediaWiki 1.29.1http://3demmethods.i2pc.es/index.php?title=2020Yoder_TimeResolved&diff=3885&oldid=prevWikiSysop: Created page with "== Citation == Yoder, N.; Jalali-Yazdi, F.; Noreng, S.; Houser, A.; Baconguis, I.; Gouaux, E. Light-coupled cryo-plunger for time-resolved cryo-EM. Journal of structural biol..."2021-02-12T07:43:15Z<p>Created page with "== Citation == Yoder, N.; Jalali-Yazdi, F.; Noreng, S.; Houser, A.; Baconguis, I.; Gouaux, E. Light-coupled cryo-plunger for time-resolved cryo-EM. Journal of structural biol..."</p>
<p><b>New page</b></p><div>== Citation ==<br />
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Yoder, N.; Jalali-Yazdi, F.; Noreng, S.; Houser, A.; Baconguis, I.; Gouaux, E. Light-coupled cryo-plunger for time-resolved cryo-EM. Journal of structural biology, 2020, 212, 107624 <br />
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== Abstract ==<br />
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Proteins are dynamic molecules that can undergo rapid conformational rearrangements in response to stimuli. These structural changes are often critical to protein function, and thus elucidating time-dependent conformational landscapes has been a long-standing goal of structural biology. To harness the power of single particle cryo-EM methods to enable 'time-resolved' structure determination, we have developed a light-coupled cryo-plunger that pairs flash-photolysis of caged ligands with rapid sample vitrification. The 'flash-plunger' consists of a high-power ultraviolet LED coupled with focusing optics and a motorized linear actuator, enabling the user to immobilize protein targets in vitreous ice within a programmable time window - as short as tens of milliseconds - after stimulus delivery. The flash-plunger is a simple, inexpensive and flexible tool to explore short-lived conformational states previously unobtainable by conventional sample preparation methods. <br />
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== Links ==<br />
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https://www.sciencedirect.com/science/article/pii/S1047847720301970<br />
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== Comments ==</div>WikiSysop