3DEM-Methods - Recent changes [en]

2013Ruskin DQE

WikiSysop — Thu, 23 Jul 2026 06:31:03 GMT

Created page with "== Citation == Ruskin, A.I., Yu, Z. and Grigorieff, N. 2013. Quantitative characterization of electron detectors for transmission electron microscopy. J. Structural biology. 184, 3 (2013), 385–393. == Abstract == A new generation of direct electron detectors for transmission electron microscopy (TEM) promises significant improvement over previous detectors in terms of their modulation transfer function (MTF) and detective quantum efficiency (DQE). However, the perfo..."

New page

== Citation ==

Ruskin, A.I., Yu, Z. and Grigorieff, N. 2013. Quantitative characterization of electron detectors for transmission electron microscopy. J. Structural biology. 184, 3 (2013), 385–393.

== Abstract ==

A new generation of direct electron detectors for transmission electron microscopy (TEM) promises significant
improvement over previous detectors in terms of their modulation transfer function (MTF) and
detective quantum efficiency (DQE). However, the performance of these new detectors needs to be carefully
monitored in order to optimize imaging conditions and check for degradation over time. We have
developed an easy-to-use software tool, FindDQE, to measure MTF and DQE of electron detectors using
images of a microscope’s built-in beam stop. Using this software, we have determined the DQE curves
of four direct electron detectors currently available: the Gatan K2 Summit, the FEI Falcon I and II, and
the Direct Electron DE-12, under a variety of total dose and dose rate conditions. We have additionally
measured the curves for the Gatan US4000 and TVIPS TemCam-F416 scintillator-based cameras. We compare
the results from our new method with published curves.

== Keywords ==

== Links ==

https://www.sciencedirect.com/science/article/pii/S1047847713002815

== Related software ==

== Related methods ==

== Comments ==

Main Page

WikiSysop — Thu, 23 Jul 2026 06:27:38 GMT

Image formation

← Older revision		Revision as of 06:27, 23 July 2026
Line 306:		Line 306:

	\| Paper		\| Paper
	\| [[~~2013Shigematsu H_Noisemodels~~]]		\| [[2013Li X K2 noisemodels]]
	\| ~~Noise models and cryo-EM drift correction~~ with ~~a direct~~-~~electron~~ camera		\| Influence of electron dose rate on electron counting images recorded with the K2 camera
			\|-

			\| Paper
			\| [[2013Ruskin_DQE]]
			\| Measurement of the DQE, camera MTF, and the noise power spectrum of cameras
	\|-		\|-

	\| Paper		\| Paper
	\| [[~~2013Li X K2 noisemodels~~]]		\| [[2013Shigematsu H_Noisemodels]]
	\| ~~Influence of~~ electron ~~dose rate on electron counting images recorded with the K2~~ camera		\| Noise models and cryo-EM drift correction with a direct-electron camera
	\|-		\|-

2026Motta Dublins

WikiSysop — Thu, 23 Jul 2026 06:19:35 GMT

Created page with "== Citation == Motta Alves, G., Andrews, T., McBean, P., Wells, T., El-Gomati, M.M., Burkhalter, S., Schulze-Briese, C., Zambon, P., McMullan, G., Henderson, R. and others 2026. The Dublin lens: a Cc= 1.0 mm objective lens intended for CryoEM at 100 keV. Methods in Microscopy. 3, 1 (2026), 19–25. == Abstract == We have designed, fabricated and tested a lens with a chromatic aberration coefficient (Cc) of 1.0 mm, a 4.0 mm pole-gap and 2.0 mm bore that is wide enough..."

New page

== Citation ==

Motta Alves, G., Andrews, T., McBean, P., Wells, T., El-Gomati, M.M., Burkhalter, S., Schulze-Briese, C., Zambon, P., McMullan, G., Henderson, R. and others 2026. The Dublin lens: a Cc= 1.0 mm objective lens intended for CryoEM at 100 keV. Methods in Microscopy. 3, 1 (2026), 19–25.

== Abstract ==

We have designed, fabricated and tested a lens with a chromatic aberration coefficient (Cc) of 1.0 mm, a 4.0 mm pole-gap and 2.0 mm bore that is wide enough to accommodate an anti-contamination system and an objective aperture. This lens extends the temporal-coherence envelope of the electron microscope beyond 2 Å, using a low-cost Schottky FEG. We hope that this lens design can be used to improve all 100 keV electron microscopes designed for single-particle electron cryomicroscopy (cryoEM).

== Keywords ==

== Links ==

https://www.degruyterbrill.com/document/doi/10.1515/mim-2025-0019/html

== Related software ==

== Related methods ==

== Comments ==

Main Page

WikiSysop — Thu, 23 Jul 2026 06:18:54 GMT

Image formation

← Older revision		Revision as of 06:18, 23 July 2026
Line 593:		Line 593:
	\| [[2026Heymann_Ewald]]		\| [[2026Heymann_Ewald]]
	\| The relationship between the Ewald sphere and exit wave explored using focal series electron micrographs		\| The relationship between the Ewald sphere and exit wave explored using focal series electron micrographs
			\|-

			\| Paper
			\| [[2026Motta_Dublins]]
			\| Dublin lens for 100kV microscopes
	\|-		\|-

2024Wang CryoSeek

WikiSysop — Wed, 22 Jul 2026 06:46:51 GMT

Created page with "== Citation == Wang, T., Li, Z., Xu, K., Huang, W., Huang, G., Zhang, Q.C. and Yan, N. 2024. CryoSeek: A strategy for bioentity discovery using cryoelectron microscopy. Proceedings of the National Academy of Sciences. 121, 42 (2024), e2417046121. == Abstract == Structural biology is experiencing a paradigm shift from targeted structural determination to structure-guided discovery of previously uncharacterized bioentities. We employed cryoelectron microscopy (cryo-EM)..."

New page

== Citation ==

Wang, T., Li, Z., Xu, K., Huang, W., Huang, G., Zhang, Q.C. and Yan, N. 2024. CryoSeek: A strategy for bioentity discovery using cryoelectron microscopy. Proceedings of the National Academy of Sciences. 121, 42 (2024), e2417046121.

== Abstract ==

Structural biology is experiencing a paradigm shift from targeted structural determination
to structure-guided
discovery of previously uncharacterized bioentities. We
employed cryoelectron microscopy (cryo-EM)
to analyze filtered water samples collected
from the Tsinghua Lotus Pond. Here, we report the structural determination and
characterization of two highly similar helical fibrils, named TLP-1a
and TLP-1b,
each
approximately 8 nm in diameter with a 15-Å
wide tunnel. These fibrils are assembled
from a similar protein protomer, whose structure was conveniently automodeled in
CryoNet. The protomer structure does not match any available experimental structures,
but shares the same fold as many predicted structures of unknown functions.
The amino-terminal
β strand of protomer n + 4 inserts into a cleft in protomer n to
complete an immunoglobulin (Ig)-like
domain. This packing mechanism, known as
donor-strand
exchange (DSE), has been observed in several bacterial pilus assemblies,
wherein the donor is protomer n + 1. Despite distinct shape and thickness, this reminiscence
suggests that TLP-1a/
b fibrils may represent uncharacterized bacterial pili. Our
study demonstrates an emerging paradigm in structural biology, where high-resolution
structural determination precedes and drives the identification and characterization of
completely unknown objects.

== Keywords ==

== Links ==

https://www.pnas.org/doi/abs/10.1073/pnas.2417046121

== Related software ==

== Related methods ==

== Comments ==

Main Page

WikiSysop — Wed, 22 Jul 2026 06:46:11 GMT

Software

← Older revision		Revision as of 06:46, 22 July 2026
Line 5,933:		Line 5,933:
	\| [[2024Vuillemot_MDSPACE]]		\| [[2024Vuillemot_MDSPACE]]
	\| MDSpace and MDTomo to analyze continuous heterogeneity		\| MDSpace and MDTomo to analyze continuous heterogeneity
			\|-

			\| Paper
			\| [[2024Wang_CryoSeek]]
			\| CryoSeek: protein identification with CryoEM, modelling and Mass spectroscopy
	\|-		\|-