View source for 2024Liu Graphene

== Citation ==

Liu, Nan / Wang, Hong-Wei. Graphene in cryo-EM specimen optimization. 2024. Current Opinion in Structural Biology, Vol. 86, p. 102823

== Abstract ==

Specimen preparation is a critical but challenging step in highresolution
cryogenic electron microscopy (cryo-EM) structural
analysis of macromolecules. In the past decade, graphene has
gained much recognition as the supporting substrate to optimize
cryo-EM specimen preparation. It improves macromolecule
embedding in ice, reduces beam-induced motion, while
imposing negligible background noise. Various types of
graphene-coated cryo-EM grids were implemented to improve
the robustness and efficiency of specimen preparation.
Graphene functionalization by different means has been
proved specifically useful in addressing challenges related to
the air-water interface (AWI), such as preferential orientation
and sample denaturation. Graphene sandwich specimen
preparation sets a new direction to explore in cryo-EM analysis
of biological specimens. In this review, we discuss the current
challenges and future prospects of graphene application in
cryo-EM analysis of macromolecules.

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== Links ==

https://www.sciencedirect.com/science/article/pii/S0959440X24000502

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