https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=1994Radermacher_Radon 2026-05-02T19:14:31Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=1994Radermacher_Radon&diff=1688&oldid=prev 2009-04-06T12:11:37Z

<p></p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Radermacher M (1994) Three-dimensional reconstruction from random<br /> projections: orientational alignment via Radon transforms.<br /> Ultramicroscopy 53:121–136<br /> <br /> [http://scholar.google.com/scholar?hl=en&amp;lr=&amp;newwindow=1&amp;cites=17429485043087722634 Cited by]<br /> <br /> == Abstract ==<br /> <br /> A method for alignment of projections with unknown projecting directions towards a three-dimensional reference has been developed. The technique has been applied to the three-dimensional reconstruction from images of a frozen-hydrated preparation of 50S ribosomal subunits from Escherichia coli recorded in the electron microscope. The algorithm as used here combines the Single Exposure Conical Reconstruction Technique (SECReT) with a three-dimensional orientation search. The algorithm allows for the refinement of a reconstruction obtained with SECReT by refinement of the true projection angles, and by the inclusion of projections with a priori unknown random orientation. With model data it is demonstrated that the algorithm works reliably even for signal-to-noise ratios lower than 1.<br /> <br /> == Keywords ==<br /> <br /> 3D Angular assignment<br /> <br /> == Links ==<br /> <br /> Article http://www.ncbi.nlm.nih.gov/pubmed/8171751<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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