https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=1997Stark_Deconvolution1997Stark Deconvolution - Revision history2026-05-24T20:35:54ZRevision history for this page on the wikiMediaWiki 1.44.2https://3demmethods.i2pc.es/index.php?title=1997Stark_Deconvolution&diff=1811&oldid=prevWikiSysop at 07:48, 29 April 20092009-04-29T07:48:01Z<p></p>
<p><b>New page</b></p><div>== Citation ==<br />
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Stark H, Orlova EV, Rinke-Appel J, Junke N, Muller F, Rodnina M,<br />
Wintermeyer W, Brimacombe R, van Heel M (1997) Arrangement<br />
of tRNAs in pre- and posttranslocational ribosomes revealed<br />
by electron cryomicroscopy. Cell 88:19–29<br />
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[http://scholar.google.com/scholar?hl=en&lr=&newwindow=1&cites=15303620716105660273 Cited by]<br />
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== Abstract ==<br />
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The three-dimensional structure of the translating 70S E. coli ribosome is presented in its two main conformations: the pretranslocational and the posttranslocational states. Using electron cryomicroscopy and angular reconstitution, structures at 20 A resolution were obtained, which, when compared with our earlier reconstruction of "empty" ribosomes, showed densities corresponding to tRNA molecules--at the P and E sites for posttranslocational ribosomes and at the A and P sites for pretranslocational ribosomes. The P-site tRNA lies directly above the bridge connecting the two ribosomal subunits, with the A-site tRNA fitted snugly against it at an angle of approximately 50 degrees, toward the L7/L12 side of the ribosome. The E-site tRNA appears to lie between the side lobe of the 30S subunit and the L1 protuberance.<br />
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== Keywords ==<br />
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CTF correction, deconvolution<br />
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== Links ==<br />
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Article http://www.ncbi.nlm.nih.gov/pubmed/9019401<br />
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== Related software ==<br />
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== Related methods ==<br />
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== Comments ==</div>WikiSysop