https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2000Jensen_TiltedCorrection 2026-05-24T19:52:24Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2000Jensen_TiltedCorrection&diff=1925&oldid=prev 2009-08-01T02:36:37Z

<p></p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Jensen, G. J. &amp; Kornberg, R. D. Defocus-gradient corrected back-projection Ultramicroscopy, 2000, 84, 57-64<br /> <br /> [http://scholar.google.com/scholar?cites=11662780474887479199 Cited by]<br /> <br /> == Abstract ==<br /> <br /> Three-dimensional reconstructions of icosahedral viruses from cryoelectron microscope images have reached resolutions where the microscope depth of field is a significant resolution-limiting factor. An analytical treatment presented here shows how the depth of field limitation can be understood as an envelope function which gradually attenuates the signal, starting well before the numerical depth of field is actually reached. A simple modification to the well-known back-projection reconstruction algorithm is described, called the defocus-gradient corrected back-projection, which computationally corrects for the contrast transfer function along a defocus gradient. Computer simulations demonstrate how the algorithm effectively eliminates the depth of field limitation.<br /> <br /> == Keywords ==<br /> <br /> CTF correction, tilt series<br /> <br /> == Links ==<br /> <br /> Article: http://www.ncbi.nlm.nih.gov/pubmed/10896140<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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