CoSS: Created page with "== Citation == Wong, H. C.; Chen, J.; Mouche, F.; Rouiller, I. & Bern, M. Model-based particle picking for cryo-electron microscopy J. Structural Biology, 2004, 145, 157-167 ..."

2013-05-20T08:23:56Z

Created page with "== Citation == Wong, H. C.; Chen, J.; Mouche, F.; Rouiller, I. & Bern, M. Model-based particle picking for cryo-electron microscopy J. Structural Biology, 2004, 145, 157-167 ..."

New page

== Citation ==

Wong, H. C.; Chen, J.; Mouche, F.; Rouiller, I. & Bern, M. Model-based particle picking for cryo-electron microscopy J. Structural Biology, 2004, 145, 157-167

== Abstract ==

We describe an algorithm for finding particle images in cryo-EM micrographs. The algorithm starts from a crude 3D map of the target particle, computed from a relatively small number of manually picked images, and then projects the map in many different directions to give synthetic 2D templates. The templates are clustered and averaged and then cross-correlated with the micrographs. A probabilistic model of the imaging process then scores cross-correlation peaks to produce the final picks. We give quantitative results on two quite different target particles: keyhole limpet hemocyanin and p97 AAA ATPase. On these particles our automatic particle picker shows human performance level, as measured by the Fourier shell correlations of 3D reconstructions.

== Keywords ==

Automatic particle picking, template based

== Links ==

http://www.ncbi.nlm.nih.gov/pubmed/15065683

== Related software ==

== Related methods ==

== Comments ==

2004Wong ParticlePicking - Revision history

CoSS: Created page with "== Citation == Wong, H. C.; Chen, J.; Mouche, F.; Rouiller, I. & Bern, M. Model-based particle picking for cryo-electron microscopy J. Structural Biology, 2004, 145, 157-167 ..."