Jjfdez at 09:10, 25 May 2009

2009-05-25T09:10:42Z

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== Citation ==

Cyrklaff M, Risco C, Fernández JJ, Jiménez MV, Estéban M, Baumeister W, Carrascosa JL. Cryo-electron tomography of vaccinia virus. Proc Natl Acad Sci U S A. 2005 102, 2772-2777.

== Abstract ==
The combination of cryo-microscopy and electron tomographic reconstruction has allowed us to determine the structure of one of the more complex viruses, intracellular mature vaccinia virus, at a resolution of 4-6 nm. The tomographic reconstruction allows us to dissect the different structural components of the viral particle, avoiding projection artifacts derived from previous microscopic observations. A surface-rendering representation revealed brick-shaped viral particles with slightly rounded edges and dimensions of approximately 360 x 270 x 250 nm. The outer layer was consistent with a lipid membrane (5-6 nm thick), below which usually two lateral bodies were found, built up by a heterogeneous material without apparent ordering or repetitive features. The internal core presented an inner cavity with electron dense coils of presumptive DNA-protein complexes, together with areas of very low density. The core was surrounded by two layers comprising an overall thickness of approximately 18-19 nm; the inner layer was consistent with a lipid membrane. The outer layer was discontinuous, formed by a periodic palisade built by the side interaction of T-shaped protein spikes that were anchored in the lower membrane and were arranged into small hexagonal crystallites. It was possible to detect a few pore-like structures that communicated the inner side of the core with the region outside the layer built by the T-shaped spike palisade.

== Keywords ==

== Links ==
http://dx.doi.org/10.1073/pnas.0409825102

== Related software ==

== Related methods ==

== Comments ==

2005Cyrklaff Thresholding - Revision history

Jjfdez at 09:10, 25 May 2009