https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2005Steven_Review 2026-05-24T20:36:36Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2005Steven_Review&diff=2737&oldid=prev 2015-11-05T10:08:54Z

<p>Created page with &quot;== Citation == Steven, A. &amp; Belnap, D. Electron microscopy and image processing: an essential tool for structural analysis of macromolecules. Curr Protoc Protein Sci, 2005, C...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Steven, A. &amp; Belnap, D. Electron microscopy and image processing: an essential tool for structural analysis of macromolecules. Curr Protoc Protein Sci, 2005, Chapter 17, Unit 17.2<br /> <br /> == Abstract ==<br /> <br /> Macromolecular electron microscopy (EM) deals with macromolecular complexes and their placement within the cell-linking the molecular and cellular worlds as a bridge between atomic-resolution X-ray crystallographic or NMR studies and lower resolution light microscopy. The amount of specimen required is typically 10(2) to 10(3) times less than for X-ray crystallography or NMR. Electron micrographs of frozen-hydrated specimens portray native structures. Computer averaging yields enhanced images with reduced noise. Three-dimensional reconstructions may be computed from multiple views. Under favorable circumstances, resolutions of 7 to 10 A are achieved. Fitting atomic-resolution coordinates of components into three-dimensional density maps gives pseudo-atomic models of a complex&#039;s structure and interactions. Time-resolved experiments describe conformational changes. Electron tomography allows reconstruction of pleiomorphic complexes and sub-cellular structures. Electron crystallography has produced near-atomic resolution models of two-dimensional arrays, notably of membrane proteins.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> http://onlinelibrary.wiley.com/doi/10.1002/0471140864.ps1702s42/abstract;jsessionid=E562C32176D0AAB1E7BD651671088FC6.f04t03<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

CoSS