https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2006Baker_segmentation 2026-05-24T20:37:18Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2006Baker_segmentation&diff=1889&oldid=prev 2009-05-22T14:43:26Z

<p></p> <p><b>New page</b></p><div>== Citation ==<br /> Baker ML, Yu Z, Chiu W, Bajaj C. Automated segmentation of molecular subunits in electron cryomicroscopy density maps. J Struct Biol. 2006 156(3):432-441. <br /> <br /> == Abstract ==<br /> Electron cryomicroscopy (cryoEM) is capable of imaging large macromolecular machines composed of multiple components. However, it is currently only possible to achieve moderate resolution at which it may be possible to computationally extract the individual components in the machine. In this work, we present application details of an automated method for detecting and segmenting the components of a large machine in an experimentally determined density map. This method is applicable to object with and without symmetry and takes advantage of global and local symmetry axes if present. We have applied this previous termsegmentationnext term algorithm to several cryoEM data sets already deposited in EMDB with various complexities, symmetries and resolutions and validated the results using manually segmented density and available structures of the components in the PDB. As such, automated previous termsegmentationnext term could become a useful tool for the analysis of the ever-increasing number of structures of macromolecular machines derived from cryoEM.<br /> <br /> == Keywords ==<br /> Segmentation; Electron cryomicroscopy; Macromolecular complexes; Structure<br /> <br /> == Links ==<br /> Article: http://dx.doi.org/10.1016/j.jsb.2006.05.013 <br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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