https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2006Messaoudi_Multiple 2026-05-24T19:52:19Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2006Messaoudi_Multiple&diff=1610&oldid=prev 2009-04-02T17:41:24Z

<p></p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Messaoudi C, Loubresse NGd, Boudier T, Dupuis-Williams P, Marco<br /> S (2006b) Multiple-axis tomography: applications to basal bodies<br /> from Paramecium tetraurelia. Biol Cell 98:415–425<br /> <br /> == Abstract ==<br /> <br /> BACKGROUND INFORMATION: Transmission electron tomography is becoming a powerful tool for studying subcellular components of cells. Classical approaches for electron tomography consist of recording images along a single-tilt axis. This approach is being improved by dual-axis reconstructions and/or high-tilt devices (tilt angle&gt;+/-60 degrees) on microscopes to compensate part of the information loss due to the &#039;missing wedge&#039; phenomena. RESULTS: In the present work we have evaluated the extension of the dual-axis technique to a multiple-axis approach, and we demonstrate a freely available plug-in for the Java-based freeware image-analysis software ImageJ. Our results from phantom and experimental data sets from Paramecium tetraurelia epon-embedded sections have shown that multiple-axis tomography achieves results equivalent to those obtained by dual-axis approach without the requirement for high-tilt devices. CONCLUSIONS: This new approach allows performance of high-resolution tomography, avoiding the need for high-tilt devices, and therefore will increase the access of electron tomography to a larger community.<br /> <br /> == Keywords ==<br /> <br /> Data collection, geometry<br /> <br /> == Links ==<br /> <br /> Article http://www.ncbi.nlm.nih.gov/pubmed/16499478<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

WikiSysop