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2013-11-11T14:41:16Z

Created page with "== Citation == Penczek, P. A.; Yang, C.; Frank, J. & Spahn, C. M. Estimation of variance in single-particle reconstruction using the bootstrap technique J. Structural Biology..."

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== Citation ==

Penczek, P. A.; Yang, C.; Frank, J. & Spahn, C. M. Estimation of variance in single-particle reconstruction using the bootstrap technique J. Structural Biology, 2006, 154, 168-183

== Abstract ==

Density maps of a molecule obtained by single-particle reconstruction from thousands of molecule projections exhibit strong changes in local definition and reproducibility, as a consequence of conformational variability of the molecule and non-stoichiometry of ligand binding. These changes complicate the interpretation of density maps in terms of molecular structure. A three-dimensional (3-D) variance map provides an effective tool to assess the structural definition in each volume element. In this work, the different contributions to the 3-D variance in a single-particle reconstruction are discussed, and an effective method for the estimation of the 3-D variance map is proposed, using a bootstrap technique of sampling. Computations with test data confirm the viability, computational efficiency, and accuracy of the method under conditions encountered in practical circumstances.

== Keywords ==

== Links ==

http://www.ncbi.nlm.nih.gov/pubmed/16510296

== Related software ==

== Related methods ==

== Comments ==

2006Penczek Bootstrap - Revision history

CoSS: Created page with "== Citation == Penczek, P. A.; Yang, C.; Frank, J. & Spahn, C. M. Estimation of variance in single-particle reconstruction using the bootstrap technique J. Structural Biology..."