https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2007Leschziner_Review 2026-05-24T19:53:19Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2007Leschziner_Review&diff=1580&oldid=prev 2009-04-02T16:45:17Z

<p></p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Leschziner AE, Nogales E (2007) Visualizing Xexibility at molecular<br /> resolution: analysis of heterogeneity in single-particle electron<br /> microscopy reconstructions. Ann Rev Biophys Biomol Struct<br /> 36:43–62<br /> <br /> == Abstract ==<br /> <br /> It is becoming increasingly clear that many macromolecules are intrinsically flexible and exist in multiple conformations in solution. Single-particle reconstruction of vitrified samples (cryo-electron microscopy, or cryo-EM) is uniquely positioned to visualize this conformational flexibility in its native state. Although heterogeneity remains a significant challenge in cryo-EM single-particle analysis, recent efforts in the field point to a future where it will be possible to tap into this rich source of biological information on a routine basis. In this article, we review the basic principles behind a few relatively new and generally applicable methods that show particular promise as tools to analyze macromolecular flexibility. We also discuss some of their recent applications to problems of biological interest.<br /> <br /> == Keywords ==<br /> <br /> Review, Heterogeneity<br /> <br /> == Links ==<br /> <br /> Article http://www.ncbi.nlm.nih.gov/pubmed/17201674<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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