https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2007Sorzano_CTF 2026-05-24T20:41:09Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2007Sorzano_CTF&diff=1503&oldid=prev 2009-04-02T10:27:15Z

<p></p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> C.O.S. Sorzano, S. Jonic, R. Núñez, N. Boisset, J.M. Carazo. Fast, robust and accurate determination of transmission electron microscopy contrast transfer function. Journal Structural Biology 160: 249-262 (2007)<br /> <br /> [http://scholar.google.com/scholar?hl=en&amp;lr=&amp;newwindow=1&amp;cites=8555944568855794248 Cited by]<br /> <br /> == Abstract ==<br /> <br /> Transmission electron microscopy, as most imaging devices, introduces optical aberrations that in the case of thin specimens are usually modeled in Fourier space by the so-called contrast transfer function (CTF). Accurate determination of the CTF is crucial for its posterior correction. Furthermore, the CTF estimation must be fast and robust if high-throughput three-dimensional electron microscopy (3DEM) studies are to be carried out. In this paper we present a robust algorithm that fits a theoretical CTF model to the power spectrum density (PSD) measured on a specific micrograph or micrograph area. Our algorithm is capable of estimating the envelope of the CTF which is absolutely needed for the correction of the CTF amplitude changes.<br /> <br /> == Keywords ==<br /> <br /> CTF estimation, image formation model<br /> <br /> == Links ==<br /> <br /> Article http://www.ncbi.nlm.nih.gov/pubmed/17911028<br /> <br /> == Related software ==<br /> <br /> Xmipp: http://xmipp.cnb.csic.es/twiki/bin/view/Xmipp/Assign_CTF<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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