https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2008Shaikh_SpiderProtocols 2026-05-24T19:51:57Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2008Shaikh_SpiderProtocols&diff=2328&oldid=prev 2013-05-20T08:09:31Z

<p>Created page with &quot;== Citation == Shaikh, T. R.; Gao, H.; Baxter, W.; Asturias, F. J.; Boisset, N.; Leith, A. &amp; Frank, J. SPIDER image processing for single-particle reconstruction of biologica...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Shaikh, T. R.; Gao, H.; Baxter, W.; Asturias, F. J.; Boisset, N.; Leith, A. &amp; Frank, J. SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs Nature protocols, 2008, 3, 1941-1974<br /> <br /> == Abstract ==<br /> <br /> This protocol describes the reconstruction of biological molecules from the electron micrographs of single particles. Computation here is performed using the image-processing software SPIDER and can be managed using a graphical user interface, termed the SPIDER Reconstruction Engine. Two approaches are described to obtain an initial reconstruction: random-conical tilt and common lines. Once an existing model is available, reference-based alignment can be used, a procedure that can be iterated. Also described is supervised classification, a method to look for homogeneous subsets when multiple known conformations of the molecule may coexist.<br /> <br /> == Keywords ==<br /> <br /> Protocols, Spider<br /> <br /> == Links ==<br /> <br /> http://www.ncbi.nlm.nih.gov/pubmed/19180078<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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