CoSS: Created page with "== Citation == Cantele, F.; Paccagnini, E.; Pigino, G.; Lupetti, P. & Lanzavecchia, S. Simultaneous alignment of dual-axis tilt series. J Struct Biol, 2010, 169, 192-199 == ..."

2013-04-01T15:04:20Z

Created page with "== Citation == Cantele, F.; Paccagnini, E.; Pigino, G.; Lupetti, P. & Lanzavecchia, S. Simultaneous alignment of dual-axis tilt series. J Struct Biol, 2010, 169, 192-199 == ..."

New page

== Citation ==

Cantele, F.; Paccagnini, E.; Pigino, G.; Lupetti, P. & Lanzavecchia, S. Simultaneous alignment of dual-axis tilt series. J Struct Biol, 2010, 169, 192-199

== Abstract ==

We present a strategy for the alignment of dual-axis tomographic series, based on reference points and
simultaneous alignment of both series. Each series is first aligned individually, an affine transformation is
determined to bring the two series in a unique reference system, and all experimental coordinates are
combined in a single system of equations. In case of severe shrinkage, a global and a local refinement
of the orientation parameters are performed to correct all minors misalignments.
The strategy is illustrated on tomographic experiments performed on sections from plastic-embedded
biological samples. The efficiency in correcting the misalignment of gold particles and in improving the
quality of the reconstruction is documented both visually and quantitatively.
In our approach every region of the tomogram is associated with its own orientation parameters and
can be eventually reconstructed with the preferred algorithm. This is convenient in the computation of
3D averages of equivalent structures. A simulation experiment is presented to show that the perfor-
mances of this approach are superior to those of the method of rotation in direct space.

== Keywords ==

Tilt-series alignment, dual axis

== Links ==

http://www.ncbi.nlm.nih.gov/pubmed/19818858

== Related software ==

== Related methods ==

== Comments ==

2010Cantele dualAlignment - Revision history

CoSS: Created page with "== Citation == Cantele, F.; Paccagnini, E.; Pigino, G.; Lupetti, P. & Lanzavecchia, S. Simultaneous alignment of dual-axis tilt series. J Struct Biol, 2010, 169, 192-199 == ..."