https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2010Yu_PPCA 2026-05-24T20:36:26Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2010Yu_PPCA&diff=3516&oldid=prev 2019-06-07T05:33:09Z

<p>Created page with &quot;== Citation == Yu, L.; Snapp, R. R.; Ruiz, T. &amp; Radermacher, M. Probabilistic principal component analysis with expectation maximization (PPCA-EM) facilitates volume classifi...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Yu, L.; Snapp, R. R.; Ruiz, T. &amp; Radermacher, M.<br /> Probabilistic principal component analysis with expectation maximization (PPCA-EM) facilitates volume classification and estimates the missing data. <br /> Journal of structural biology, 2010 , 171 , 18-30 <br /> <br /> == Abstract ==<br /> <br /> We have developed a new method for classifying 3D reconstructions with missing data obtained by electron microscopy techniques. The method is based on principal component analysis (PCA) combined with expectation maximization. The missing data, together with the principal components, are treated as hidden variables that are estimated by maximizing a likelihood function. PCA in 3D is similar to PCA for 2D image analysis. A lower dimensional subspace of significant features is selected, into which the data are projected, and if desired, subsequently classified. In addition, our new algorithm estimates the missing data for each individual volume within the lower dimensional subspace. Application to both a large model data set and cryo-electron microscopy experimental data demonstrates the good performance of the algorithm and illustrates its potential for studying macromolecular assemblies with continuous conformational variations.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.ncbi.nlm.nih.gov/pubmed/20385241<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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