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== Citation ==

Glaeser R. M., Typke D., Tiemeijer P. C., Pulokas J., Chang A. Review: Precise Beam-Tilt Alignment And Collimation Are Required To Minimize The Phase Error Associated With Coma In High-Resolution Cryo-EM

. J Struct Biol. 2011 April;174(1):1-10.

== Abstract ==

Electron microscopy at a resolution of 0.4 nm or better requires more careful adjustment of the
illumination than is the case at a resolution of 0.8 nm. The use of current-axis alignment is not
always sufficient, for example, to avoid the introduction of large phase errors, at higher resolution,
due to axial coma. In addition, one must also ensure that off-axis coma does not corrupt the data
quality at the higher resolution. We particularly emphasize that the standard CTF correction does
not account for the phase error associated with coma. We explain the cause of both axial coma and
the typically most troublesome component of off-axis coma in terms of the well-known shift of the
electron diffraction pattern relative to the optical axis that occurs when the illumination is not
parallel to the axis. We review the experimental conditions under which coma causes unacceptably
large phase errors, and we discuss steps that can be taken when setting up the conditions of
illumination, so as to ensure that neither axial nor off-axis coma is a problem.

== Keywords ==

Beam alignment; coma; phase error

== Links ==

http://www.sciencedirect.com/science/article/pii/S1047847710003825

== Related software ==

== Related methods ==

== Comments ==

2011Glaeser Coma - Revision history

Javier: Created page with "== Citation == Glaeser R. M., Typke D., Tiemeijer P. C., Pulokas J., Chang A. Review: Precise Beam-Tilt Alignment And Collimation Are Required To Minimize The Phase Error Ass..."