https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2012Devaux_Protocol 2026-05-24T20:35:35Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2012Devaux_Protocol&diff=2243&oldid=prev 2012-12-17T10:30:17Z

<p>Created page with &quot;== Citation == Z. Devaux, C.O.S. Sorzano, S. Jonic. Processing of Transmission Electron Microscopy Images for Single-Particle Analysis of Macromolecular Complexes. In P. M. C...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Z. Devaux, C.O.S. Sorzano, S. Jonic. Processing of Transmission Electron Microscopy Images for Single-Particle Analysis of Macromolecular Complexes. In P. M. Conn (Ed.), Laboratory Methods in Cell Biology (pp. 295–310). Elsevier Inc. (2012)<br /> <br /> == Abstract ==<br /> <br /> Three-dimensional (3D) structure of a wide range of biological macromolecular<br /> assemblies can be computed from two-dimensional images collected by transmission<br /> electron microscopy. This information integrated with other structural data (e.g.,<br /> from X-ray crystallography or nuclear magnetic resonance) helps structural biologists<br /> understand the function of macromolecular complexes. Single-particle analysis<br /> (SPA) is a method used for studies of complexes whose structure and dynamics can<br /> be analyzed in isolation. To reconstruct the 3D structure, SPA methods use a large<br /> number of images of randomly oriented individual complexes. When the angular<br /> distribution of single-particle orientations samples Fourier space completely and the<br /> population is structurally homogeneous, a resolution of the reconstruction of 0.4-1<br /> nm can be achieved. Such high resolutions are possible thanks to the high number of<br /> images and the correction of the Contrast Transfer Function (CTF) of the microscope.<br /> One of the standard SPA approaches is the refinement of a preliminary 3D<br /> model using iterative projection matching combined with CTF correction. We<br /> describe a protocol for the refinement of a preliminary model using CTF correction<br /> by Wiener filtering of volumes from focal series of experimental images. This protocol<br /> combines potentially best features of two other protocols proposed in the field.<br /> <br /> == Keywords ==<br /> <br /> Protocols, Single Particle Analysis, High-throughput<br /> <br /> == Links ==<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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