https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2012Sorzano_Review2012Sorzano Review - Revision history2026-05-24T20:20:43ZRevision history for this page on the wikiMediaWiki 1.44.2https://3demmethods.i2pc.es/index.php?title=2012Sorzano_Review&diff=2241&oldid=prevCoSS: Created page with "== Citation == C.O.S. Sorzano, J. M. de la Rosa Trevín, J. Otón, J. J. Vega, J. Cuenca, A. Zaldívar-Peraza, J. Gómez-Blanco, J. Vargas, A. Quintana, R. Marabini, J. M. Ca..."2012-12-17T10:24:43Z<p>Created page with "== Citation == C.O.S. Sorzano, J. M. de la Rosa Trevín, J. Otón, J. J. Vega, J. Cuenca, A. Zaldívar-Peraza, J. Gómez-Blanco, J. Vargas, A. Quintana, R. Marabini, J. M. Ca..."</p>
<p><b>New page</b></p><div>== Citation ==<br />
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C.O.S. Sorzano, J. M. de la Rosa Trevín, J. Otón, J. J. Vega, J. Cuenca, A. Zaldívar-Peraza, J. Gómez-Blanco, J. Vargas, A. Quintana, R. Marabini, J. M. Carazo. Semiautomatic, high-throughput, high-resolution protocol for three-dimensional reconstruction of Single Particles in Electron Microscopy. Nanoimaging: Methods and Protocols. Methods in Molecular Biology, 950: 171-193. Eds. Alioscka Sousa, Michael Kruhlak. Humana Press. (2012)<br />
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== Abstract ==<br />
<br />
In this chapter we describe the steps needed for reconstructing the three-dimensional structure of a<br />
macromolecular complex starting from its projections collected in electron micrographs. The concepts are<br />
shown through the use of Xmipp 3.0, a software suite specifically designed for the image processing of<br />
biological structures imaged with electron or X-ray microscopy. We illustrate the image processing workflow<br />
by applying it to the images of Bovine Papilloma virus published in Wolf et al. (Proc Natl Acad Sci USA<br />
107:6298–6303, 2010). We show that in the case of high-quality, homogeneous datasets with a priori<br />
knowledge about the initial volume, we can have a high-resolution 3D reconstruction in less than 1 day<br />
using a computer cluster with only 32 processors.<br />
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== Keywords ==<br />
<br />
Single Particle Analysis, High-throughput, Xmipp<br />
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== Links ==<br />
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== Related software ==<br />
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== Related methods ==<br />
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== Comments ==</div>CoSS