2013Shigematsu H Noisemodels - Revision history

Jose Maria Carazo: /* Keywords */

2013-09-11T14:15:52Z

Keywords

← Older revision		Revision as of 14:15, 11 September 2013
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	== Keywords ==		== Keywords ==

	~~On the use of DNA origami gold particles as internal handedness starndar~~		"Hot pixel noise" in Direct Detectors (DE-12)

	== Links ==		== Links ==

Jose Maria Carazo: /* Abstract */

2013-09-11T14:13:32Z

Abstract

← Older revision		Revision as of 14:13, 11 September 2013
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	== Abstract ==		== Abstract ==
			Blurring due to specimen-holder drift is a common occurrence in cryo-EM images. Cameras employing active-pixel sensors are capable of high frame rates such that a single low-dose exposure can be acquired as a series of frames. In this paper we consider the possibility of tracking and compensating for overall drift in typical single-particle specimens through the analysis of frame sequences. A problem that arises in tracking through cross-correlation of frames obtained with the DE-12 camera from Direct Electron LLC is the presence of "hot-pixel noise". This random pattern of bright pixels is highly correlated among frames. We show how a model of this noise can be employed to greatly reduce its effects. A filter function is derived that optimizes the tracking of image shifts by cross-correlation, and we demonstrate the tracking of specimen drift in typical cryo-EM specimens.

	== Keywords ==		== Keywords ==

Jose Maria Carazo: /* Abstract */

2013-09-11T14:09:48Z

Abstract

← Older revision		Revision as of 14:09, 11 September 2013
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	== Abstract ==		== Abstract ==

	The apparent handedness of an EM-tomography reconstruction depends on a number of conventions andc an be confused in many ways. As the number of different hardware and software combinations being used for electron tomography continue to climb, and the reconstructions being produced reach higherand higher resolutions, the need to verify the hand of the results has increased. Here we enumerate various steps in a typical tomography experiment that affect handedness and show that DNA origami gold nanoparticle helices can be used as convenient and fail-safe handedness standards.

	== Keywords ==		== Keywords ==

Blanca Benitez: Created page with "== Citation == Shigematsu H, Sigworth FJ.Noise models and cryo-EM drift correction with a direct-electron camera.U ltramicroscopy. 2013 Aug;131:61-9. == Abstract == The app..."

2013-08-14T16:13:45Z

Created page with "== Citation == Shigematsu H, Sigworth FJ.Noise models and cryo-EM drift correction with a direct-electron camera.U ltramicroscopy. 2013 Aug;131:61-9. == Abstract == The app..."

New page

== Citation ==

Shigematsu H, Sigworth FJ.Noise models and cryo-EM drift correction with a direct-electron camera.U ltramicroscopy. 2013 Aug;131:61-9.

== Abstract ==

The apparent handedness of an EM-tomography reconstruction depends on a number of conventions andc an be confused in many ways. As the number of different hardware and software combinations being used for electron tomography continue to climb, and the reconstructions being produced reach higherand higher resolutions, the need to verify the hand of the results has increased. Here we enumerate various steps in a typical tomography experiment that affect handedness and show that DNA origami gold nanoparticle helices can be used as convenient and fail-safe handedness standards.

== Keywords ==

On the use of DNA origami gold particles as internal handedness starndar

== Links ==

http://www.ncbi.nlm.nih.gov/pubmed/23748163

== Related software ==

== Related methods ==

== Comments ==