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2013-08-16T13:26:21Z

Created page with "== Citation == Shigematsu, H. & Sigworth, F. J. Noise models and cryo-EM drift correction with a direct-electron camera. Ultramicroscopy, 2013, 131, 61-69 == Abstract == Bl..."

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== Citation ==

Shigematsu, H. & Sigworth, F. J. Noise models and cryo-EM drift correction with a direct-electron camera. Ultramicroscopy, 2013, 131, 61-69

== Abstract ==

Blurring due to specimen-holder drift is a common occurrence in cryo-EM images. Cameras employing active-pixel sensors are capable of high frame rates such that a single low-dose exposure can be acquired as a series of frames. In this paper we consider the possibility of tracking and compensating for overall drift in typical single-particle specimens through the analysis of frame sequences. A problem that arises in tracking through cross-correlation of frames obtained with the DE-12 camera from Direct Electron LLC is the presence of "hot-pixel noise". This random pattern of bright pixels is highly correlated among frames. We show how a model of this noise can be employed to greatly reduce its effects. A filter function is derived that optimizes the tracking of image shifts by cross-correlation, and we demonstrate the tracking of specimen drift in typical cryo-EM specimens.

== Keywords ==

== Links ==

http://www.ncbi.nlm.nih.gov/pubmed/23748163

== Related software ==

== Related methods ==

== Comments ==

2013Shigematsu Movies - Revision history

CoSS: Created page with "== Citation == Shigematsu, H. & Sigworth, F. J. Noise models and cryo-EM drift correction with a direct-electron camera. Ultramicroscopy, 2013, 131, 61-69 == Abstract == Bl..."