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Created page with "== Citation == Han, R.; Zhang, F.; Wan, X.; Fernández, J.-J.; Sun, F. & Liu, Z. A marker-free automatic alignment method based on scale-invariant features. Journal of struct..."

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== Citation ==

Han, R.; Zhang, F.; Wan, X.; Fernández, J.-J.; Sun, F. & Liu, Z. A marker-free automatic alignment method based on scale-invariant features. Journal of structural biology, 2014, 186, 167-180

== Abstract ==

In electron tomography, alignment accuracy is critical for high-resolution reconstruction. However, the automatic alignment of a tilt series without fiducial markers remains a challenge. Here, we propose a new alignment method based on Scale-Invariant Feature Transform (SIFT) for marker-free alignment. The method covers the detection and localization of interest points (features), feature matching, feature tracking and optimization of projection parameters. The proposed method implements a highly reliable matching strategy and tracking model to detect a huge number of feature tracks. Furthermore, an incremental bundle adjustment method is devised to tolerate noise data and ensure the accurate estimation of projection parameters. Our method was evaluated with a number of experimental data, and the results exhibit an improved alignment accuracy comparable with current fiducial marker alignment and subsequent higher resolution of tomography.

== Keywords ==

== Links ==

http://www.sciencedirect.com/science/article/pii/S1047847714000458

== Related software ==

== Related methods ==

== Comments ==

2014Han Automatic - Revision history

CoSS: Created page with "== Citation == Han, R.; Zhang, F.; Wan, X.; Fernández, J.-J.; Sun, F. & Liu, Z. A marker-free automatic alignment method based on scale-invariant features. Journal of struct..."