CoSS: Created page with "== Citation == Elmlund, D. & Elmlund, H. Cryogenic electron microscopy and single-particle analysis. Annu Rev Biochem, 2015, 84, 499-517 == Abstract == About 20 years ago, ..."

2015-09-30T13:37:44Z

Created page with "== Citation == Elmlund, D. & Elmlund, H. Cryogenic electron microscopy and single-particle analysis. Annu Rev Biochem, 2015, 84, 499-517 == Abstract == About 20 years ago, ..."

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== Citation ==

Elmlund, D. & Elmlund, H. Cryogenic electron microscopy and single-particle analysis. Annu Rev Biochem, 2015, 84, 499-517

== Abstract ==

About 20 years ago, the first three-dimensional (3D) reconstructions at subnanometer (10-Å) resolution of an icosahedral virus assembly were obtained by cryogenic electron microscopy (cryo-EM) and single-particle analysis. Since then, thousands of structures have been determined to resolutions ranging from 30 Å to near atomic (4 Å). Almost overnight, the recent development of direct electron detectors and the attendant improvement in analysis software have advanced the technology considerably. Near-atomic-resolution reconstructions can now be obtained, not only for megadalton macromolecular complexes or highly symmetrical assemblies but also for proteins of only a few hundred kilodaltons. We discuss the developments that led to this breakthrough in high-resolution structure determination by cryo-EM and point to challenges that lie ahead.

== Keywords ==

== Links ==

http://www.ncbi.nlm.nih.gov/pubmed/25747402

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2015Elmlund Review - Revision history

CoSS: Created page with "== Citation == Elmlund, D. & Elmlund, H. Cryogenic electron microscopy and single-particle analysis. Annu Rev Biochem, 2015, 84, 499-517 == Abstract == About 20 years ago, ..."