https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2015Grant_Anisotropic 2026-05-24T21:06:45Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2015Grant_Anisotropic&diff=2784&oldid=prev 2015-12-29T09:05:38Z

<p>Created page with &quot;== Citation == Grant, T. &amp; Grigorieff, N. Automatic estimation and correction of anisotropic magnification distortion in electron microscopes. J Struct Biol, 2015, 192, 204-2...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Grant, T. &amp; Grigorieff, N. Automatic estimation and correction of anisotropic magnification distortion in electron microscopes. J Struct Biol, 2015, 192, 204-208<br /> <br /> == Abstract ==<br /> <br /> We demonstrate a significant anisotropic magnification distortion, found on an FEI Titan Krios microscope and affecting magnifications commonly used for data acquisition on a Gatan K2 Summit detector. We describe a program (mag_distortion_estimate) to automatically estimate anisotropic magnification distortion from a set of images of a standard gold shadowed diffraction grating. We also describe a program (mag_distortion_correct) to correct for the estimated distortion in collected images. We demonstrate that the distortion present on the Titan Krios microscope limits the resolution of a set of rotavirus VP6 images to ∼7 Å, which increases to ∼3 Å following estimation and correction of the distortion. We also use a 70S ribosome sample to demonstrate that in addition to affecting resolution, magnification distortion can also interfere with the classification of heterogeneous data.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> http://www.sciencedirect.com/science/article/pii/S1047847715300447<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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