https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2015Kuijper_FEI 2026-05-24T20:20:47Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2015Kuijper_FEI&diff=2750&oldid=prev 2015-11-19T15:10:37Z

<p>Created page with &quot;== Citation == Kuijper, M.; van Hoften, G.; Janssen, B.; Geurink, R.; De Carlo, S.; Vos, M.; van Duinen, G.; van Haeringen, B. &amp; Storms, M. FEI&#039;s direct electron detector dev...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Kuijper, M.; van Hoften, G.; Janssen, B.; Geurink, R.; De Carlo, S.; Vos, M.; van Duinen, G.; van Haeringen, B. &amp; Storms, M. FEI&#039;s direct electron detector developments: Embarking on a revolution in cryo-TEM. J Struct Biol, 2015, 192, 179-187<br /> <br /> == Abstract ==<br /> <br /> In early 2011 FEI Company launched the &quot;Falcon&quot;, its first commercial direct electron detector product intended for application in 3-D electron microscopy in the life sciences. In this paper we discuss the principle of direct electron detection and its implementation in Falcon cameras. We describe the signal formation in the sensor and its impact on the detection quantum efficiency (DQE) of the sensor. Insights into the signal formation led us to improved camera designs. Three significant improvements are discussed. (1) Back thinning of the sensor. This is implemented in the second-generation Falcon (Falcon 2), where the sensor thickness is reduced to 50μm, and in the latest generation Falcon 3 detector with further back-thinning down to 30μm. (2) The introduction of electron counting, a signal processing technology implemented in Falcon 3. (3) Dose fractionation mode, which allows the user to access intermediate results during the illumination of the sample.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> http://www.ncbi.nlm.nih.gov/pubmed/26431895<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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