CoSS: Created page with "== Citation == Rohou, A. & Grigorieff, N. CTFFIND4: Fast and accurate defocus estimation from electron micrographs. J Struct Biol, 2015, 192, 216-221 == Abstract == CTFFIND..."

2015-11-23T12:11:41Z

Created page with "== Citation == Rohou, A. & Grigorieff, N. CTFFIND4: Fast and accurate defocus estimation from electron micrographs. J Struct Biol, 2015, 192, 216-221 == Abstract == CTFFIND..."

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== Citation ==

Rohou, A. & Grigorieff, N. CTFFIND4: Fast and accurate defocus estimation from electron micrographs. J Struct Biol, 2015, 192, 216-221

== Abstract ==

CTFFIND is a widely-used program for the estimation of objective lens defocus parameters from transmission electron micrographs. Defocus parameters are estimated by fitting a model of the microscope's contrast transfer function (CTF) to an image's amplitude spectrum. Here we describe modifications to the algorithm which make it significantly faster and more suitable for use with images collected using modern technologies such as dose fractionation and phase plates. We show that this new version preserves the accuracy of the original algorithm while allowing for higher throughput. We also describe a measure of the quality of the fit as a function of spatial frequency and suggest this can be used to define the highest resolution at which CTF oscillations were successfully modeled.

== Keywords ==

== Links ==

http://www.ncbi.nlm.nih.gov/pubmed/26278980

== Related software ==

== Related methods ==

== Comments ==

2015Rohou CTFFind4 - Revision history

CoSS: Created page with "== Citation == Rohou, A. & Grigorieff, N. CTFFIND4: Fast and accurate defocus estimation from electron micrographs. J Struct Biol, 2015, 192, 216-221 == Abstract == CTFFIND..."