https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2015Scheres_SemiAutoPicking 2026-05-24T21:06:03Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2015Scheres_SemiAutoPicking&diff=3277&oldid=prev 2018-05-10T08:54:29Z

<p>Created page with &quot;== Citation == Scheres, S. H. (2015). Semi-automated selection of cryo-EM particles in RELION-1.3. Journal of structural biology, 189(2), 114-122. == Abstract == The selectio...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> Scheres, S. H. (2015). Semi-automated selection of cryo-EM particles in RELION-1.3. Journal of structural biology, 189(2), 114-122.<br /> <br /> == Abstract ==<br /> The selection of particles suitable for high-resolution cryo-EM structure determination from noisy micrographs may represent a tedious and time-consuming step. Here, a semi-automated particle selection procedure is presented that has been implemented within the open-source software RELION. At the heart of the procedure lies a fully CTF-corrected template-based picking algorithm, which is supplemented by a fast sorting algorithm and reference-free 2D class averaging to remove false positives. With only limited user-interaction, the proposed procedure yields results that are comparable to manual particle selection. Together with an improved graphical user interface, these developments further contribute to turning RELION from a stand-alone refinement program into a convenient image processing pipeline for the entire single-particle approach.<br /> <br /> == Keywords ==<br /> Electron cryo-microscopySingle-particle analysisAutomated particle picking<br /> <br /> == Links ==<br /> https://www.sciencedirect.com/science/article/pii/S1047847714002615<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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