https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2016Kim_SAXS 2026-05-24T22:01:03Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2016Kim_SAXS&diff=2951&oldid=prev 2016-10-17T11:58:01Z

<p>Created page with &quot;== Citation == Kim, J. S.; Afsari, B. &amp; Chirikjian, G. S. Cross-Validation of Data Compatibility Between Small Angle X-ray Scattering and Cryo-Electron Microscopy. Journal of...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Kim, J. S.; Afsari, B. &amp; Chirikjian, G. S. Cross-Validation of Data Compatibility Between Small Angle X-ray Scattering and Cryo-Electron Microscopy. Journal of computational biology : a journal of computational molecular cell biology, 2016<br /> <br /> == Abstract ==<br /> <br /> Cryo-electron microscopy (EM) and small angle X-ray scattering (SAXS) are two different data acquisition modalities often used to glean information about the structure of large biomolecular complexes in their native states. A SAXS experiment is generally considered fast and easy but unveils the structure at very low resolution, whereas a cryo-EM experiment needs more extensive preparation and postacquisition computation to yield a three-dimensional (3D) density map at higher resolution. In certain applications, we may need to verify whether the data acquired in the SAXS and cryo-EM experiments correspond to the same structure (e.g., before reconstructing the 3D density map in EM). In this article, a simple and fast method is proposed to verify the compatibility of the SAXS and EM experimental data. The method is based on averaging the two-dimensional correlation of EM images and the Abel transform of the SAXS data. Orientational preferences are known to exist in cryo-EM experiments, and we also consider these effects on our method. The results are verified on simulations of conformational states of large biomolecular complexes.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.ncbi.nlm.nih.gov/pubmed/27710115<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

CoSS