https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2016Passmore_Review2016Passmore Review - Revision history2026-05-24T21:05:56ZRevision history for this page on the wikiMediaWiki 1.44.2https://3demmethods.i2pc.es/index.php?title=2016Passmore_Review&diff=2891&oldid=prevCoSS: Created page with "== Citation == Passmore, L. & Russo, C. J. Methods in Enzymology. The Resolution Revolution: Recent Advances In cryoEM Specimen preparation for high-resolution cryo-EM Academ..."2016-08-31T05:03:26Z<p>Created page with "== Citation == Passmore, L. & Russo, C. J. Methods in Enzymology. The Resolution Revolution: Recent Advances In cryoEM Specimen preparation for high-resolution cryo-EM Academ..."</p>
<p><b>New page</b></p><div>== Citation ==<br />
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Passmore, L. & Russo, C. J. Methods in Enzymology. The Resolution Revolution: Recent Advances In cryoEM Specimen preparation for high-resolution cryo-EM Academic Press, 2016, 51-86<br />
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== Abstract ==<br />
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Imaging a material with electrons at near-atomic resolution requires a thin specimen<br />
that is stable in the vacuum of the transmission electron microscope. For biological samples,<br />
this comprises a thin layer of frozen aqueous solution containing the biomolecular<br />
complex of interest. The process of preparing a high-quality specimen is often the limiting<br />
step in the determination of structures by single-particle electron cryomicroscopy<br />
(cryo-EM). Here, we describe a systematic approach for going from a purified biomolecular<br />
complex in aqueous solution to high-resolution electron micrographs that are suitable<br />
for 3D structure determination. This includes a series of protocols for the<br />
preparation of vitrified specimens on various supports, including all-gold and graphene.<br />
We also describe techniques for troubleshooting when a preparation fails to yield suitable<br />
specimens, and common mistakes to avoid during each part of the process. Finally,<br />
we include recommendations for obtaining the highest quality micrographs from prepared<br />
specimens with current microscope, detector, and support technology.<br />
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== Keywords ==<br />
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== Links ==<br />
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http://www.sciencedirect.com/science/article/pii/S0076687916300295<br />
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== Related software ==<br />
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== Related methods ==<br />
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== Comments ==</div>CoSS