CoSS: Created page with "== Citation == Thompson, R. F.; Walker, M.; Siebert, C. A.; Muench, S. P. & Ranson, N. A. An introduction to sample preparation and imaging by cryo-electron microscopy for st..."

2016-08-26T15:59:22Z

Created page with "== Citation == Thompson, R. F.; Walker, M.; Siebert, C. A.; Muench, S. P. & Ranson, N. A. An introduction to sample preparation and imaging by cryo-electron microscopy for st..."

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== Citation ==

Thompson, R. F.; Walker, M.; Siebert, C. A.; Muench, S. P. & Ranson, N. A. An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology. Methods, 2016, 100, 3-15

== Abstract ==

Transmission electron microscopy (EM) is a versatile technique that can be used to image biological specimens ranging from intact eukaryotic cells to individual proteins >150 kDa. There are several strategies for preparing samples for imaging by EM, including negative staining and cryogenic freezing. In the last few years, cryo-EM has undergone a ‘resolution revolution’, owing to both advances in imaging hardware, image processing software, and improvements in sample preparation, leading to growing number of researchers using cryo-EM as a research tool. However, cryo-EM is still a rapidly growing field, with unique challenges. Here, we summarise considerations for imaging of a range of specimens from macromolecular complexes to cells using EM.

== Keywords ==

== Links ==

http://www.sciencedirect.com/science/article/pii/S1046202316300330

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== Related methods ==

== Comments ==

2016Thompson Sample - Revision history

CoSS: Created page with "== Citation == Thompson, R. F.; Walker, M.; Siebert, C. A.; Muench, S. P. & Ranson, N. A. An introduction to sample preparation and imaging by cryo-electron microscopy for st..."