https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2016Vinothkumar_Review 2026-06-13T12:11:44Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2016Vinothkumar_Review&diff=2849&oldid=prev 2016-08-08T05:53:03Z

<p>Created page with &quot;== Citation == Vinothkumar, K. R. &amp; Henderson, R. Single particle electron cryomicroscopy: trends, Issues and future perspective. Quarterly Reviews of Biophysics, 2016, 49, e...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Vinothkumar, K. R. &amp; Henderson, R. Single particle electron cryomicroscopy: trends, Issues and future perspective. Quarterly Reviews of Biophysics, 2016, 49, e13: 1-25<br /> <br /> == Abstract ==<br /> <br /> There has been enormous progress during the last few years in the determination of three-dimensional biological structures by<br /> single particle electron cryomicroscopy (cryoEM), allowing maps to be obtained with higher resolution and from fewer images than required<br /> previously. This is due principally to the introduction of a new type of direct electron detector that has 2- to 3-fold higher detective quantum<br /> efficiency than available previously, and to the improvement of the computational algorithms for image processing. In spite of the great strides<br /> that have been made, quantitative analysis shows that there are still significant gains to be made provided that the problems associated with<br /> image degradation can be solved, possibly by minimising beam-induced specimen movement and charge build up during imaging. If this can<br /> be achieved, it should be possible to obtain near atomic resolution structures of smaller single particles, using fewer images and resolving more<br /> conformational states than at present, thus realising the full potential of the method. The recent popularity of cryoEM for molecular structure<br /> determination also highlights the need for lower cost microscopes, so we encourage development of an inexpensive, 100 keV electron cryo-<br /> microscope with a high-brightness field emission gun to make the method accessible to individual groups or institutions that cannot afford the<br /> investment and running costs of a state-of-the-art 300 keV installation. A key requisite for successful high-resolution structure determination<br /> by cryoEM includes interpretation of images and optimising the biochemistry and grid preparation to obtain nicely distributed macromolecules of interest. We thus include in this review a gallery of cryoEM micrographs that shows illustrative examples of single particle images of large and small macromolecular complexes.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> http://journals.cambridge.org/download.php?file=%2FQRB%2FQRB49%2FS0033583516000068a.pdf&amp;code=a01cce20afe7c69674f024857fd4885c<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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