https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2016Wang_DeepPicker 2026-05-24T21:06:32Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2016Wang_DeepPicker&diff=3285&oldid=prev 2018-05-10T09:17:11Z

<p>Created page with &quot;== Citation == Wang, F., Gong, H., Liu, G., Li, M., Yan, C., Xia, T., ... &amp; Zeng, J. (2016). DeepPicker: A deep learning approach for fully automated particle picking in cryo-...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> Wang, F., Gong, H., Liu, G., Li, M., Yan, C., Xia, T., ... &amp; Zeng, J. (2016). DeepPicker: A deep learning approach for fully automated particle picking in cryo-EM. Journal of structural biology, 195(3), 325-336.<br /> <br /> == Abstract ==<br /> Particle picking is a time-consuming step in single-particle analysis and often requires significant interventions from users, which has become a bottleneck for future automated electron cryo-microscopy (cryo-EM). Here we report a deep learning framework, called DeepPicker, to address this problem and fill the current gaps toward a fully automated cryo-EM pipeline. DeepPicker employs a novel cross-molecule training strategy to capture common features of particles from previously-analyzed micrographs, and thus does not require any human intervention during particle picking. Tests on the recently-published cryo-EM data of three complexes have demonstrated that our deep learning based scheme can successfully accomplish the human-level particle picking process and identify a sufficient number of particles that are comparable to those picked manually by human experts. These results indicate that DeepPicker can provide a practically useful tool to significantly reduce the time and manual effort spent in single-particle analysis and thus greatly facilitate high-resolution cryo-EM structure determination. DeepPicker is released as an open-source program, which can be downloaded from https://github.com/nejyeah/DeepPicker-python.<br /> <br /> == Keywords ==<br /> Cryo-EMParticle pickingAutomationDeep learning<br /> <br /> == Links ==<br /> https://www.sciencedirect.com/science/article/pii/S1047847716301472<br /> <br /> == Related software ==<br /> https://github.com/nejyeah/DeepPicker-python<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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