https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2016Zhang_DNANG 2026-05-24T20:40:39Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2016Zhang_DNANG&diff=4852&oldid=prev 2024-11-12T09:28:57Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 09:28, 12 November 2024</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Citation ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Citation ==</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">Molecular Organization </del>of <del style="font-weight: bold; text-decoration: none;">the Early Stages of Nucleosome Phase Separation Visualized </del>by <del style="font-weight: bold; text-decoration: none;">Cryo</del>-Electron Tomography, <del style="font-weight: bold; text-decoration: none;">Meng </del>Zhang, <del style="font-weight: bold; text-decoration: none;">César Díaz-Celis, Bibiana Onoa, Cristhian Cañari-Chumpitaz</del>, <del style="font-weight: bold; text-decoration: none;">Katherinne I</del>. <del style="font-weight: bold; text-decoration: none;">Requejo</del>, <del style="font-weight: bold; text-decoration: none;">Jianfang Liu</del>, <del style="font-weight: bold; text-decoration: none;">Michael Vien</del>, <del style="font-weight: bold; text-decoration: none;">Eva Nogales</del>, Gang Ren, <del style="font-weight: bold; text-decoration: none;">and Carlos Bustamante, Molecular Cell</del>, (<del style="font-weight: bold; text-decoration: none;">2022</del>), <del style="font-weight: bold; text-decoration: none;">18;82(16)</del>:<del style="font-weight: bold; text-decoration: none;">3000-3014</del>.<del style="font-weight: bold; text-decoration: none;">e9; PMID: 35907400 DOI</del>: 10.<del style="font-weight: bold; text-decoration: none;">1016/j.molcel.2022.06.032</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">3D Structural Dynamics and Fluctuations </ins>of <ins style="font-weight: bold; text-decoration: none;">DNA-Nanogold Conjugates </ins>by <ins style="font-weight: bold; text-decoration: none;">Individual</ins>-<ins style="font-weight: bold; text-decoration: none;">Particle </ins>Electron Tomography, <ins style="font-weight: bold; text-decoration: none;">Lei </ins>Zhang, <ins style="font-weight: bold; text-decoration: none;">Dongsheng Lei</ins>, <ins style="font-weight: bold; text-decoration: none;">Jessica M</ins>. <ins style="font-weight: bold; text-decoration: none;">Smith</ins>, <ins style="font-weight: bold; text-decoration: none;">Huimin Tong</ins>, <ins style="font-weight: bold; text-decoration: none;">Xing Zhang</ins>, <ins style="font-weight: bold; text-decoration: none;">Zhuoyang Lu</ins>, <ins style="font-weight: bold; text-decoration: none;">Paul Alivisatos and </ins>Gang Ren, <ins style="font-weight: bold; text-decoration: none;">Nature Communications</ins>, (<ins style="font-weight: bold; text-decoration: none;">2016</ins>), <ins style="font-weight: bold; text-decoration: none;">7</ins>:<ins style="font-weight: bold; text-decoration: none;">11083</ins>. <ins style="font-weight: bold; text-decoration: none;">doi</ins>: 10.<ins style="font-weight: bold; text-decoration: none;">1038</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Abstract ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Abstract ==</div></td></tr> <tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l6">Line 6:</td> <td colspan="2" class="diff-lineno">Line 6:</td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Keywords ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Keywords ==</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">Cryo-ET, single </del>molecule structure, individual-particle <del style="font-weight: bold; text-decoration: none;">cryo-</del>electron tomography, IPET, <del style="font-weight: bold; text-decoration: none;">nucleosome array, phase transition, aggregation</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Single </ins>molecule structure, individual-particle electron tomography, IPET, <ins style="font-weight: bold; text-decoration: none;">DNA nanogold conjugate</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Paper links ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Paper links ==</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>https://<del style="font-weight: bold; text-decoration: none;">pubmed</del>.<del style="font-weight: bold; text-decoration: none;">ncbi</del>.<del style="font-weight: bold; text-decoration: none;">nlm.nih.gov</del>/<del style="font-weight: bold; text-decoration: none;">35907400</del>/</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>https://<ins style="font-weight: bold; text-decoration: none;">www</ins>.<ins style="font-weight: bold; text-decoration: none;">nature</ins>.<ins style="font-weight: bold; text-decoration: none;">com</ins>/<ins style="font-weight: bold; text-decoration: none;">articles</ins>/<ins style="font-weight: bold; text-decoration: none;">ncomms11083</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Related video ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Related video ==</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>https://www.<del style="font-weight: bold; text-decoration: none;">cell</del>.com/<del style="font-weight: bold; text-decoration: none;">cms/10.1016/j.molcel.2022.06.032/attachment/530f75ed-d615-46af-bdf6-255b34acf813/mmc2.mp4</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>https://www.<ins style="font-weight: bold; text-decoration: none;">youtube</ins>.com/<ins style="font-weight: bold; text-decoration: none;">watch?v=lQrbmg9ry90</ins></div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">https://www.cell.com/cms/10.1016/j.molcel.2022.06.032/attachment/d2ae50b4-cafd-40f1-95c6-355322774031/mmc3.mp4</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">https://www.cell.com/cms/10.1016/j.molcel.2022.06.032/attachment/bc9def6c-803f-4938-83d7-c4fd72610fc8/mmc4.mp4</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Comments ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Comments ==</div></td></tr> </table>

Garygangren https://3demmethods.i2pc.es/index.php?title=2016Zhang_DNANG&diff=4850&oldid=prev 2024-11-12T09:24:05Z

<p>Created page with &quot;== Citation == Molecular Organization of the Early Stages of Nucleosome Phase Separation Visualized by Cryo-Electron Tomography, Meng Zhang, César Díaz-Celis, Bibiana Onoa, Cristhian Cañari-Chumpitaz, Katherinne I. Requejo, Jianfang Liu, Michael Vien, Eva Nogales, Gang Ren, and Carlos Bustamante, Molecular Cell, (2022), 18;82(16):3000-3014.e9; PMID: 35907400 DOI: 10.1016/j.molcel.2022.06.032 == Abstract == It has been proposed that the intrinsic property of nucleosom...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> Molecular Organization of the Early Stages of Nucleosome Phase Separation Visualized by Cryo-Electron Tomography, Meng Zhang, César Díaz-Celis, Bibiana Onoa, Cristhian Cañari-Chumpitaz, Katherinne I. Requejo, Jianfang Liu, Michael Vien, Eva Nogales, Gang Ren, and Carlos Bustamante, Molecular Cell, (2022), 18;82(16):3000-3014.e9; PMID: 35907400 DOI: 10.1016/j.molcel.2022.06.032<br /> <br /> == Abstract ==<br /> It has been proposed that the intrinsic property of nucleosome arrays to undergo liquid-liquid phase separation (LLPS) in vitro is responsible for chromatin domain organization in vivo. However, understanding nucleosomal LLPS has been hindered by the challenge to characterize the structure of the resulting heterogeneous condensates. We used cryo-electron tomography and deep-learning-based 3D reconstruction/segmentation to determine the molecular organization of condensates at various stages of LLPS. We show that nucleosomal LLPS involves a two-step process: a spinodal decomposition process yielding irregular condensates, followed by their unfavorable conversion into more compact, spherical nuclei that grow into larger spherical aggregates through accretion of spinodal materials or by fusion with other spherical condensates. Histone H1 catalyzes more than 10-fold the spinodal-to-spherical conversion. We propose that this transition involves exposure of nucleosome hydrophobic surfaces causing modified inter-nucleosome interactions. These results suggest a physical mechanism by which chromatin may transition from interphase to metaphase structures.<br /> <br /> == Keywords ==<br /> Cryo-ET, single molecule structure, individual-particle cryo-electron tomography, IPET, nucleosome array, phase transition, aggregation<br /> <br /> == Paper links ==<br /> https://pubmed.ncbi.nlm.nih.gov/35907400/<br /> <br /> == Related video ==<br /> https://www.cell.com/cms/10.1016/j.molcel.2022.06.032/attachment/530f75ed-d615-46af-bdf6-255b34acf813/mmc2.mp4<br /> https://www.cell.com/cms/10.1016/j.molcel.2022.06.032/attachment/d2ae50b4-cafd-40f1-95c6-355322774031/mmc3.mp4<br /> https://www.cell.com/cms/10.1016/j.molcel.2022.06.032/attachment/bc9def6c-803f-4938-83d7-c4fd72610fc8/mmc4.mp4<br /> <br /> == Comments ==</div>

Garygangren