https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2017Arnold_BlottingFree 2026-05-24T19:53:17Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2017Arnold_BlottingFree&diff=3085&oldid=prev 2017-05-11T20:53:55Z

<p>Created page with &quot;== Citation == Arnold, S. A.; Albiez, S.; Bieri, A.; Syntychaki, A.; Adaixo, R.; McLeod, R. A.; Goldie, K. N.; Stahlberg, H. &amp; Braun, T. Blotting-free and lossless cryo-elect...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Arnold, S. A.; Albiez, S.; Bieri, A.; Syntychaki, A.; Adaixo, R.; McLeod, R. A.; Goldie, K. N.; Stahlberg, H. &amp; Braun, T. Blotting-free and lossless cryo-electron microscopy grid preparation from nanoliter-sized protein samples and single-cell extracts. Journal of structural biology, 2017, 197, 220-226<br /> <br /> == Abstract ==<br /> <br /> We present a sample preparation method for cryo-electron microscopy (cryo-EM) that requires only 3-20nL of sample to prepare a cryo-EM grid, depending on the protocol used. The sample is applied and spread on the grid by a microcapillary. The procedure does not involve any blotting steps, and real-time monitoring allows the water film thickness to be assessed and decreased to an optimum value prior to vitrification. We demonstrate that the method is suitable for high-resolution cryo-EM and will enable alternative electron microscopy approaches, such as single-cell visual proteomics.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> http://www.sciencedirect.com/science/article/pii/S104784771630243X<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

CoSS