https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2017Elmlund_Review 2026-06-13T12:37:53Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2017Elmlund_Review&diff=3300&oldid=prev 2018-05-27T08:30:53Z

<p>Created page with &quot;== Citation == Elmlund, D.; Le, S. N. &amp; Elmlund, H. High-resolution cryo-EM: the nuts and bolts. Current opinion in structural biology, 2017 , 46 , 1-6 == Abstract == Cryo...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Elmlund, D.; Le, S. N. &amp; Elmlund, H. High-resolution cryo-EM: the nuts and bolts. Current opinion in structural biology, 2017 , 46 , 1-6 <br /> <br /> == Abstract ==<br /> <br /> Cryogenic electron microscopy (cryo-EM) and single-particle analysis now enables the determination of high-resolution structures of macromolecular assemblies that have resisted X-ray crystallography and other approaches. Successful high-resolution structure determination by cryo-EM always depends on the quality of the protein sample. While structural heterogeneity remains a key challenge for cryo-EM, it also represents a rare opportunity to study the intrinsic conformational flexibility of macromolecular assemblies. Here, we review the key technological advancements that have made this &#039;resolution revolution&#039; possible and give a concise overview of the technical challenges that needed to be overcome to allow high-resolution structure determination.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.ncbi.nlm.nih.gov/pubmed/28342396<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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