CoSS: Created page with "== Citation == He, J.; Hsieh, C.; Wu, Y.; Schmelzer, T.; Wang, P.; Lin, Y.; Marko, M. & Sui, H. Cryo-FIB specimen preparation for use in a cartridge-type cryo-TEM. Journal of..."

2017-07-20T06:03:12Z

Created page with "== Citation == He, J.; Hsieh, C.; Wu, Y.; Schmelzer, T.; Wang, P.; Lin, Y.; Marko, M. & Sui, H. Cryo-FIB specimen preparation for use in a cartridge-type cryo-TEM. Journal of..."

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== Citation ==

He, J.; Hsieh, C.; Wu, Y.; Schmelzer, T.; Wang, P.; Lin, Y.; Marko, M. & Sui, H. Cryo-FIB specimen preparation for use in a cartridge-type cryo-TEM. Journal of structural biology, 2017

== Abstract ==

Cryo-electron tomography (cryo-ET) is a well-established technique for studying 3D structural details of
subcellular macromolecular complexes and organelles in their nearly native context in the cell. A primary
limitation of the application of cryo-ET is the accessible specimen thickness, which is less than the diameters
of almost all eukaryotic cells. It has been shown that focused ion beam (FIB) milling can be used to
prepare thin, distortion-free lamellae of frozen biological material for high-resolution cryo-ET.
Commercial cryosystems are available for cryo-FIB specimen preparation, however re-engineering and
additional fixtures are often essential for reliable results with a particular cryo-FIB and cryotransmission
electron microscope (cryo-TEM). Here, we describe our optimized protocol and modified
instrumentation for cryo-FIB milling to produce thin lamellae and subsequent damage-free cryotransfer
of the lamellae into our cartridge-type cryo-TEM.

== Keywords ==

== Links ==

http://www.sciencedirect.com/science/article/pii/S1047847717300916

== Related software ==

== Related methods ==

== Comments ==

2017He FIB - Revision history

CoSS: Created page with "== Citation == He, J.; Hsieh, C.; Wu, Y.; Schmelzer, T.; Wang, P.; Lin, Y.; Marko, M. & Sui, H. Cryo-FIB specimen preparation for use in a cartridge-type cryo-TEM. Journal of..."