https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2017McLeod_Zorro 2026-05-24T21:06:42Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2017McLeod_Zorro&diff=3067&oldid=prev 2017-05-11T13:44:28Z

<p>Created page with &quot;== Citation == McLeod, R. A.; Kowal, J.; Ringler, P. &amp; Stahlberg, H. Robust image alignment for cryogenic transmission electron microscopy. Journal of structural biology, 201...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> McLeod, R. A.; Kowal, J.; Ringler, P. &amp; Stahlberg, H. Robust image alignment for cryogenic transmission electron microscopy. Journal of structural biology, 2017, 197, 279-293<br /> <br /> == Abstract ==<br /> <br /> Cryo-electron microscopy recently experienced great improvements in structure resolution due to direct electron detectors with improved contrast and fast read-out leading to single electron counting. High frames rates enabled dose fractionation, where a long exposure is broken into a movie, permitting specimen drift to be registered and corrected. The typical approach for image registration, with high shot noise and low contrast, is multi-reference (MR) cross-correlation. Here we present the software package Zorro, which provides robust drift correction for dose fractionation by use of an intensity-normalized cross-correlation and logistic noise model to weight each cross-correlation in the MR model and filter each cross-correlation optimally. Frames are reliably registered by Zorro with low dose and defocus. Methods to evaluate performance are presented, by use of independently-evaluated even- and odd-frame stacks by trajectory comparison and Fourier ring correlation. Alignment of tiled sub-frames is also introduced, and demonstrated on an example dataset. Zorro source code is available at github.com/CINA/zorro.<br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> http://www.sciencedirect.com/science/article/pii/S1047847716302520<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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