https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2018Anoshina_Simulation2018Anoshina Simulation - Revision history2026-05-24T20:15:36ZRevision history for this page on the wikiMediaWiki 1.44.2https://3demmethods.i2pc.es/index.php?title=2018Anoshina_Simulation&diff=3482&oldid=prevWikiSysop: Created page with "== Citation == Anoshina, N.; Sagindykov, T. & Sorokin, D. A Method for Generation of Synthetic 2D and 3D Cryo-EM Images. Programming and Computer Software, Springer, 2018, 44..."2019-04-17T17:12:46Z<p>Created page with "== Citation == Anoshina, N.; Sagindykov, T. & Sorokin, D. A Method for Generation of Synthetic 2D and 3D Cryo-EM Images. Programming and Computer Software, Springer, 2018, 44..."</p>
<p><b>New page</b></p><div>== Citation ==<br />
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Anoshina, N.; Sagindykov, T. & Sorokin, D. A Method for Generation of Synthetic 2D and 3D Cryo-EM Images. Programming and Computer Software, Springer, 2018, 44, 240-247<br />
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== Abstract ==<br />
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Cryo-electron microscopy (cryo-EM) is widely used in structural biology for resolving 3D models of particles with Angstrom resolution. The most popular techniques for such high-resolution model reconstruction are single-particle cryo-EM and cryo-electron tomography (cryo-ET). The cornerstone of both techniques is the registration of images: 2D images in cryo-EM and 3D images in cryo-ET. There are several registration methods for 2D and 3D cryo-EM images; however, it is hard to evaluate these methods due to the lack of ground truth for real data. Moreover, evaluation of image registration methods on real data is complicated by a high level of noise. In this paper, we propose image synthesis methods for generating realistic 2D single-particle cryo-EM images and 3D cryo-ET subtomogram images. The proposed algorithms model the artifacts typical of the real EM image acquisition pipeline: EM-specific noise, missing wedge effect, 2D projection, and contrast transfer function. We also present some examples of the 2D and 3D synthetic images generated.<br />
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== Keywords ==<br />
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== Links ==<br />
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https://link.springer.com/article/10.1134/S0361768818040023<br />
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== Comments ==</div>WikiSysop