WikiSysop: Created page with "== Citation == Bartesaghi, A.; Aguerrebere, C.; Falconieri, V.; Banerjee, S.; Earl, L. A.; Zhu, X.; Grigorieff, N.; Milne, J. L. S.; Sapiro, G.; Wu, X. & Subramaniam, S. Atom..."

2018-05-27T09:03:53Z

Created page with "== Citation == Bartesaghi, A.; Aguerrebere, C.; Falconieri, V.; Banerjee, S.; Earl, L. A.; Zhu, X.; Grigorieff, N.; Milne, J. L. S.; Sapiro, G.; Wu, X. & Subramaniam, S. Atom..."

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== Citation ==

Bartesaghi, A.; Aguerrebere, C.; Falconieri, V.; Banerjee, S.; Earl, L. A.; Zhu, X.; Grigorieff, N.; Milne, J. L. S.; Sapiro, G.; Wu, X. & Subramaniam, S. Atomic Resolution Cryo-EM Structure of β-Galactosidase. Structure, 2018

== Abstract ==

The advent of direct electron detectors has enabled the routine use of single-particle cryo-electron microscopy (EM) approaches to determine structures of a variety of protein complexes at near-atomic resolution. Here, we report the development of methods to account for local variations in defocus and beam-induced drift, and the implementation of a data-driven dose compensation scheme that significantly improves the extraction of high-resolution information recorded during exposure of the specimen to the electron beam. These advances enable determination of a cryo-EM density map for β-galactosidase bound to the inhibitor phenylethyl β-D-thiogalactopyranoside where the ordered regions are resolved at a level of detail seen in X-ray maps at ∼ 1.5 Å resolution. Using this density map in conjunction with constrained molecular dynamics simulations provides a measure of the local flexibility of the non-covalently bound inhibitor and offers further opportunities for structure-guided inhibitor design.

== Keywords ==

== Links ==

https://www.cell.com/structure/abstract/S0969-2126(18)30129-1

== Related software ==

== Related methods ==

== Comments ==

2018Bartesaghi Refinement - Revision history

WikiSysop: Created page with "== Citation == Bartesaghi, A.; Aguerrebere, C.; Falconieri, V.; Banerjee, S.; Earl, L. A.; Zhu, X.; Grigorieff, N.; Milne, J. L. S.; Sapiro, G.; Wu, X. & Subramaniam, S. Atom..."