https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2018Hattne_RadiationDamage2018Hattne RadiationDamage - Revision history2026-05-24T21:06:49ZRevision history for this page on the wikiMediaWiki 1.44.2https://3demmethods.i2pc.es/index.php?title=2018Hattne_RadiationDamage&diff=3315&oldid=prevWikiSysop: Created page with "== Citation == Hattne, J.; Shi, D.; Glynn, C.; Zee, C.-T.; Gallagher-Jones, M.; Martynowycz, M. W.; Rodriguez, J. A. & Gonen, T. Analysis of Global and Site-Specific Radiatio..."2018-05-27T09:10:27Z<p>Created page with "== Citation == Hattne, J.; Shi, D.; Glynn, C.; Zee, C.-T.; Gallagher-Jones, M.; Martynowycz, M. W.; Rodriguez, J. A. & Gonen, T. Analysis of Global and Site-Specific Radiatio..."</p>
<p><b>New page</b></p><div>== Citation ==<br />
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Hattne, J.; Shi, D.; Glynn, C.; Zee, C.-T.; Gallagher-Jones, M.; Martynowycz, M. W.; Rodriguez, J. A. & Gonen, T. Analysis of Global and Site-Specific Radiation Damage in Cryo-EM. Structure, 2018 , 26 , 759-766.e4 <br />
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== Abstract ==<br />
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Micro-crystal electron diffraction (MicroED) combines the efficiency of electron scattering with diffraction to allow structure determination from nano-sized crystalline samples in cryoelectron microscopy (cryo-EM). It has been used to solve structures of a diverse set of biomolecules and materials, in some cases to sub-atomic resolution. However, little is known about the damaging effects of the electron beam on samples during such measurements. We assess global and site-specific damage from electron radiation on nanocrystals of proteinase K and of a prion hepta-peptide and find that the dynamics of electron-induced damage follow well-established trends observed in X-ray crystallography. Metal ions are perturbed, disulfide bonds are broken, and acidic side chains are decarboxylated while the diffracted intensities decay exponentially with increasing exposure. A better understanding of radiation damage in MicroED improves our assessment and processing of all types of cryo-EM data.<br />
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== Links ==<br />
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https://www.cell.com/structure/abstract/S0969-2126(18)30125-4<br />
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== Comments ==</div>WikiSysop