https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2018Neumann_validation 2026-05-24T20:15:57Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2018Neumann_validation&diff=3259&oldid=prev 2018-04-30T10:04:18Z

<p>Created page with &quot;== Citation == Neumann, P.; Dickmanns, A. &amp; Ficner, R. Validating Resolution Revolution. Structure, 2018 == Abstract == Recent advances in instrumentation and image-process...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Neumann, P.; Dickmanns, A. &amp; Ficner, R. Validating Resolution Revolution. Structure, 2018 <br /> <br /> == Abstract ==<br /> Recent advances in instrumentation and image-processing software have resulted in a resolution revolution in cryo-electron microscopy (cryo-EM) and a surge in the popularity of this technique. However, despite technical progress and hundreds of structures determined so far, development of standards assessing the agreement between the cryo-EM map and the respective model has fallen behind. Here we establish a validation procedure evaluating this agreement and applied it to a set of 565 cryo-EM structures. Analysis of the results revealed that three-quarters of the validated structures exhibit moderate or low agreement between the map and the corresponding model, mostly due to limited structural features possessed by these maps. Model re-refinement significantly improved the agreement for only one-fifth of the structures, reaffirming the necessity to re-evaluate map resolution. The presented procedure provides an approach to re-estimate the resolution of cryo-EM map areas interpreted by the model. <br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.cell.com/structure/abstract/S0969-2126(18)30084-4<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

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