https://3demmethods.i2pc.es/index.php?action=history&feed=atom&title=2018Noble_Reducing 2026-06-13T12:12:46Z Revision history for this page on the wiki MediaWiki 1.44.2 https://3demmethods.i2pc.es/index.php?title=2018Noble_Reducing&diff=3445&oldid=prev 2019-02-04T21:08:27Z

<p>Created page with &quot;== Citation == Noble, A. J.; Wei, H.; Dandey, V. P.; Zhang, Z.; Tan, Y. Z.; Potter, C. S. and Carragher, B. Reducing effects of particle adsorption to the air-water interface...&quot;</p> <p><b>New page</b></p><div>== Citation ==<br /> <br /> Noble, A. J.; Wei, H.; Dandey, V. P.; Zhang, Z.; Tan, Y. Z.; Potter, C. S. and Carragher, B. Reducing effects of particle adsorption to the air-water interface in cryo-EM. Nature methods, 2018, 15, 793-795 <br /> <br /> == Abstract ==<br /> <br /> Most protein particles prepared in vitreous ice for single-particle cryo-electron microscopy (cryo-EM) are adsorbed to air-water or substrate-water interfaces, which can cause the particles to adopt preferred orientations. By using a rapid plunge-freezing robot and nanowire grids, we were able to reduce some of the deleterious effects of the air-water interface by decreasing the dwell time of particles in thin liquid films. We demonstrated this by using single-particle cryo-EM and cryo-electron tomography (cryo-ET) to examine hemagglutinin, insulin receptor complex, and apoferritin. <br /> <br /> == Keywords ==<br /> <br /> == Links ==<br /> <br /> https://www.nature.com/articles/s41592-018-0139-3<br /> <br /> == Related software ==<br /> <br /> == Related methods ==<br /> <br /> == Comments ==</div>

WikiSysop